RFLP ANALYSIS AND LINKAGE MAPPING IN SOLANUM-TUBEROSUM
RFLP ANALYSIS AND LINKAGE MAPPING IN SOLANUM-TUBEROSUM
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DOI：
10.1007/bf00299755
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发表时间：
1989-01-01
影响因子：
5.4
通讯作者：
SALAMINI, F
中科院分区：
文献类型：
--
作者：
GEBHARDT, C;RITTER, E;SALAMINI, F
使用 168 个马铃薯探针（包括随机基因组和 cDNA 序列以及已知功能的特征性马铃薯基因）分析了 38 个二倍体马铃薯品系的形态学和农艺异质性集合的限制性片段长度多态性 (RFLP)。使用四种切割限制酶和变性聚丙烯酰胺凝胶上 250 至 2,000 个碱基的片段分离范围可以检测少数核苷酸的 RFLP。通过该系统，90% 的测试探针显示出有用的多态性，其中 95% 的探针在使用两种或全部三种酶时具有多态性。平均而言，80% 的探针在 38 个品系的所有成对比较中提供信息，最低为 49%，最高为 95%。确定每个品系相对于彼此的杂合性百分比，并表明 F1 群体中的直接分离分析对于大多数组合来说应该是可行的。通过涉及 38 个品系中的一对的回交，构建了具有 141 个基因座的 RFLP 连锁图谱，覆盖马铃薯基因组的 690 cMorgan。
A morphologically and agronomically heterogeneous collection of 38 diploid potato lines was analysed for restriction fragment length polymorphisms (RFLPs) with 168 potato probes, including random genomic and cDNA sequences as well as characterized potato genes of known function. The use of four cutter restriction enzymes and a fragment separation range from 250 to 2,000 bases on denaturing polyacrylamide gels allowed the detection of RFLPs of a few nucleotides. With this system, 90% of all probes tested showed useful polymorphism, and 95% of those were polymorphic with two or all three enzymes used. On the average, 80% of the probes were informative in all pairwise comparisons of the 38 lines with a minimum of 49% and a maximum of 95%. The percentage of heterozygosity was determined relative to each other for each line and indicated that direct segregation analysis in F1 populations should be feasible for most combinations. From a backcross involving one pair of the 38 lines, a RFLP linkage map with 141 loci was constructed, covering 690 cMorgan of the Solanum tuberosum genome.