Olfactory ensheathing cells: Attractant of neural progenitor migration to olfactory bulb
Olfactory ensheathing cells: Attractant of neural progenitor migration to olfactory bulb
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DOI：
10.1002/glia.20957
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发表时间：
2010-04
期刊：
影响因子：
6.2
通讯作者：
Yanling Zhu;Li Cao;Z. Su;Lifang Mu;Yi-min Yuan;Liang Gao;Y. Qiu;Cheng He
中科院分区：
文献类型：
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作者：
Yanling Zhu;Li Cao;Z. Su;Lifang Mu;Yi-min Yuan;Liang Gao;Y. Qiu;Cheng He
嗅鞘细胞（Olfactory ensheathing cells，OECs）是来源于嗅基板的神经胶质细胞，在从嗅上皮向嗅球迁移的过程中包裹嗅轴突，主要位于嗅神经层。嗅鞘细胞移植作为一种有前途的治疗轴突损伤的实验方法已被广泛研究，但其在嗅球发育中的作用却知之甚少。在这项研究中，我们研究了嗅鞘细胞对吻侧迁移流（RMS）中神经祖细胞迁移的影响。最初，神经球迁移试验表明，OEC条件培养基以浓度依赖性方式促进祖细胞从RMS神经球迁移。此外，在RMS外植体附近共培养OEC导致不同发育阶段的外植体的不对称迁移。然而，OECs可以影响迁移的距离不超过1.5 mm。最后，切片分析，模拟在体内的情况下，显示OECs对RMS神经祖细胞具有化学吸引活性。总之，这些结果表明，嗅鞘细胞通过释放扩散因子吸引RMS中的神经祖细胞，并且很可能嗅鞘细胞主要影响嗅球中的径向迁移，但在发育过程中RMS体内的切向迁移不受影响。这表明嗅鞘细胞在嗅觉发育中的一种先前未知的功能和一种靶向RMS细胞的新机制。© 2010 Wiley利斯公司
Olfactory ensheathing cells (OECs) are the glial cells that derive from the olfactory placode, envelop olfactory axons in the course of migration from the olfactory epithelium to the olfactory bulb and reside primarily in the olfactory nerve layer. OECs transplantation as a promising experimental therapy for axonal injuries has been intensively studied; however, little is known about their roles in olfactory bulb development. In this study, we examined the effects of OECs on the migration of neural progenitors in rostral migratory stream (RMS). Initially, the neurosphere migration assay showed that OEC‐conditioned medium promoted progenitors to migrate from RMS neurospheres in a concentration dependent manner. Moreover, co‐culturing OECs nearby the RMS explants led to asymmetric migration of explants in different developing stages. However, OECs could influence the migration in a distance not further than 1.5 mm. Finally, slice assay that mimic the circumstance in vivo revealed that OECs had a chemoattractive activity on RMS neural progenitors. Together, these results demonstrate that OECs attract neural progenitors in RMS through the release of diffusible factors and it is likely that OECs mainly influence radial migration in the olfactory bulb but not tangential migration of the RMS invivo during development. This suggests a previously unknown function for OECs in olfactory development and a novel mechanism underlying the targeting of RMS cells. © 2010 Wiley‐Liss, Inc.