Involvement of Polo-like kinase 1 in MEK1/2-regulated spindle formation during mouse oocyte meiosis
Involvement of Polo-like kinase 1 in MEK1/2-regulated spindle formation during mouse oocyte meiosis
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DOI：
10.4161/cc.7.12.6019
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发表时间：
2008-06
期刊：
影响因子：
4.3
通讯作者：
Bo Xiong;Shao‐Chen Sun;Sheng-Li Lin;Mo Li;Bao-Zeng Xu;Yingchun Ouyang;Yi Hou;Dayuan Chen;Qing-Yuan Sun
中科院分区：
文献类型：
--
作者：
Bo Xiong;Shao‐Chen Sun;Sheng-Li Lin;Mo Li;Bao-Zeng Xu;Yingchun Ouyang;Yi Hou;Dayuan Chen;Qing-Yuan Sun
我们最近的研究表明，MEK1/2是卵母细胞减数分裂过程中微管组织和纺锤体形成的关键调节因子。在本研究中，我们发现Plk1在GVBD后微管开始组织的各个减数分裂阶段与p-MEK1/2共定位。此外，Plk1能够与p-MEK1/2在I中期小鼠卵母细胞提取物中共免疫沉淀，进一步证实了它们的物理相互作用。紫杉醇处理的卵母细胞显示出许多细胞质母粒，其中Plk1和p-MEK1/2都存在，表明它们可能在卵母细胞减数分裂期间在MTOCs中参与无丝胞体纺锤体的形成。nocodazole对微管的解聚导致纺锤体完全解体，但Plk1仍然与p-MEK1/2相关，积聚在染色体附近。更重要的是，当p-MEK1/2活性被U0126阻断时，Plk1失去了在纺锤极的正常定位，这可能是导致mek1 /2抑制卵母细胞纺锤体异常的最重要因素之一。综上所述，这些数据表明Plk1和MEK1/2以相同的途径调节纺锤体的形成，并且在小鼠卵母细胞减数分裂成熟过程中，Plk1参与了MEK1/2调节的纺锤体组装。
Our recent studies have shown that MEK1/2 is a critical regulator of microtubule organization and spindle formation during oocyte meiosis. In the present study, we found that Plk1 colocalized with p-MEK1/2 at various meoiotic stages after GVBD when microtubule began to organize. Also, Plk1 was able to coimmunoprecipitate with p-MEK1/2 in metaphase I stage mouse oocyte extracts, further confirming their physical interaction. Taxol-treated oocytes exhibited a number of cytoplasmic asters, in which both Plk1 and p-MEK1/2 were present, indicating that they might be complexed to participate in the acentrosomal spindle formation at the MTOCs during oocyte meiosis. Depolymerization of microtubules by nocodazole resulted in the complete disassembly of spindles, but Plk1 remained associated with p-MEK1/2, accumulating in the vicinity of chromosomes. More importantly, when p-MEK1/2 activity was blocked by U0126, Plk1 lost its normal localization at the spindle poles, which might be one of the most vital factors causing the abnormal spindles in MEK1/2-inhibited oocytes. Taken together, these data indicate that Plk1 and MEK1/2 regulate the spindle formation in the same pathway and that Plk1 is involved in MEK1/2-regulated spindle assembly during mouse oocyte meiotic maturation.