High-performance liquid chromatography with tandem mass spectrometry for the determination of nine hallucinogenic 25-NBOMe designer drugs in urine specimens.
High-performance liquid chromatography with tandem mass spectrometry for the determination of nine hallucinogenic 25-NBOMe designer drugs in urine specimens.
复制标题
采用高效液相色谱串联质谱法测定尿液样本中的 9 种致幻性 25-NBOMe 设计药物。
DOI：
10.1093/jat/bku005
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发表时间：
2014
影响因子：
2.5
通讯作者：
Poklis,Alphonse
中科院分区：
文献类型：
--
作者：
Poklis,JustinL;Clay,DeborahJ;Poklis,Alphonse
本文建立了高效液相色谱-四极柱质谱（HPLC-MS-MS）方法，用于鉴定和定量人尿中9种羟色胺5-HT2A受体激动剂致幻物质，这些致幻物质来自于一类新的甲氧基苯乙胺（NBOMe） n -甲氧基苄基衍生物：25H-NBOMe、2CC-NBOMe、25I-NBF、25D-NBOMe、25B-NBOMe、2CT-NBOMe、25I-NBMD、25G-NBOMe和25I-NBOMe。该检测方法是为弗吉尼亚联邦大学临床和法医毒理学实验室开发的，用于筛查急诊部门的标本，以应对弗吉尼亚爆发的n -苄基苯乙胺衍生物滥用和过量病例。使用FASt™固相萃取柱从尿液标本中快速提取nome衍生物。根据法医毒理学科学工作组（SWGTOX）推荐的线性度、定量下限、检测下限、准确性/偏置、精密度、稀释完整性、残留、选择性、绝对回收率、离子抑制和稳定性等方面的验证来确定分析性能。9种分析物的线性范围为1 ~ 100 ng/mL。NBOMe衍生物测定的偏差为86-116%，在测定的线性范围内变异系数<14%。采用该方法在患者尿液标本中检测了四种不同的nome衍生物。
We present a high-performance liquid chromatography triple quadrupole mass spectrometry (HPLC–MS-MS) method for the identification and quantification of nine serotonin 5-HT2A receptor agonist hallucinogenic substances from a new class ofN-methoxybenzyl derivatives of methoxyphenylethylamine (NBOMe) designer drugs in human urine: 25H-NBOMe, 2CC-NBOMe, 25I-NBF, 25D-NBOMe, 25B-NBOMe, 2CT-NBOMe, 25I-NBMD, 25G-NBOMe and 25I-NBOMe. This assay was developed for the Virginia Commonwealth University Clinical and Forensic Toxicology laboratory to screen emergency department specimens in response to an outbreak ofN-benzyl-phenethylamine derivative abuse and overdose cases in Virginia. The NBOMe derivatives were rapidly extracted from the urine specimens by use of FASt™ solid-phase extraction columns. Assay performance was determined as recommended for validation by the Scientific Working Group for Forensic Toxicology (SWGTOX) for linearity, lower limit of quantification, lower limit of detection, accuracy/bias, precision, dilution integrity, carryover, selectivity, absolute recovery, ion suppression and stability. Linearity was verified to be from 1 to 100 ng/mL for each of the nine analytes. The bias determined for the NBOMe derivatives was 86–116% with a <14% coefficient of variation over the linear range of the assay. Four different NBOMe derivatives were detected using the presented method in patient urine specimens.