BATCH PURIFICATION OF OVOMUCOID AND CHARACTERIZATION OF PURIFIED PRODUCT
BATCH PURIFICATION OF OVOMUCOID AND CHARACTERIZATION OF PURIFIED PRODUCT
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DOI：
10.1021/bi00777a006
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发表时间：
1971-01-01
期刊：
影响因子：
2.9
通讯作者：
DONOVAN, JW
中科院分区：
文献类型：
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作者：
DAVIS, JG;MAPES, CJ;DONOVAN, JW
^虽然卵泡样物质已通过许多方法从蛋清中分离出来，但Lineweaver和Murray（1947）三氯乙酸-丙酮法可能是近年来应用最广泛的方法。这是通常用于商业生产的方法（见Melamed， 1966, and Montreuil et al., 1965）。通过这种方法获得的类卵粘液制剂，包括那些来自商业来源的制剂，可能含有高达20%的其他蛋清蛋白作为杂质。其中鉴定的有溶菌酶（Tomimatsu et al., 1966; Donovan, 1967）、卵细胞抑制剂（Feeney et al., 1963; Tomimatsu et al., 1966; Ket-terer 's, 1965）、糖蛋白（Montreuil et al., 1965）、卵清蛋白，可能还有黄蛋白（见下文）。Melamed（1966）总结了纯化Lineweaver-Murray（1947）卵泡样蛋白的方法。对离子交换纤维素进行色谱是一种普遍接受的方法，尽管在对羧甲基纤维素进行色谱时，对类卵粘液的唾液酸残基在低pH值下的稳定性提出了质疑(Rhodes et al., 1960; Chatterjee and Montgomery，
^ Although ovomucoid has been separated from egg white by a number of methods, the Lineweaver and Murray (1947) trichloroacetic acid-acetone procedure has probably been the most widely used in recent years. It is the method used commonly for commercial production (see Melamed, 1966, and Montreuil et al., 1965, for reviews). Preparations of ovomucoid obtained by this method, including those from commercial sources, may contain up to 20% of other egg-white proteins as impurities. Among those identified are lysozyme (Tomimatsu et al., 1966; Donovan, 1967), ovo-inhibitor (Feeney et al., 1963; Tomimatsu et al., 1966; Ket-terer’s, 1965), glycoprotein (Montreuil et al., 1965), ovalbumins, and probably flavoprotein (see below). Melamed (1966) has summarized the methods used to purify Lineweaver-Murray (1947) ovomucoid. Chromatography on ion-exchange celluloses is the generallyaccepted procedure even though doubt has been raisedconcerning the stability of the sialic acid residues of ovomucoid at thelow pH encountered during chromatography on carboxymethyl-cellulose (Rhodes et al., 1960; Chatterjee and Montgomery,