Transcription profile analysis reveals that zygotic division results in uneven distribution of specific transcripts in apical/basal cells of tobacco.
Transcription profile analysis reveals that zygotic division results in uneven distribution of specific transcripts in apical/basal cells of tobacco.
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转录谱分析表明，合子分裂导致烟草顶/基细胞中特定转录物的分布不均匀。
DOI：
10.1371/journal.pone.0015971
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发表时间：
2011-01-07
期刊：
影响因子：
3.7
通讯作者：
Sun M
中科院分区：
文献类型：
--
作者：
Ma L;Xin H;Qu L;Zhao J;Yang L;Zhao P;Sun M
高等植物中的不对称合子分裂导致顶细胞和基底细胞的形成。这两种胚胎细胞具有不同的形态和细胞发育命运。有人提出，不均匀分布的细胞命运决定因素和/或不同的细胞转录谱可能是其不同命运的根本原因。然而，由于技术限制，这些假设都没有令人信服的支持。使用激光控制显微切割，我们分离了顶端细胞和基底细胞，并构建了细胞类型特异性 cDNA 文库。转录本概况分析揭示了转录本组成的差异。 PCR和qPCR分析证实所选胚胎发生相关基因的转录本是细胞类型优先分布的。发现受精卵中存在的一些转录物明显存在于顶端或基底细胞中。合子细胞分裂后也发现了细胞类型特异性的从头转录。因此，我们发现顶端细胞和基底细胞之间存在转录多样性。不对称合子分裂导致一些胚胎发生相关转录本在双细胞原胚中分布不均匀，这表明一些特定转录本在顶端或基底细胞中的差异分布可能涉及引导两种细胞类型走向不同的发育命运。
Asymmetric zygotic division in higher plants results in the formation of an apical cell and a basal cell. These two embryonic cells possess distinct morphologies and cell developmental fates. It has been proposed that unevenly distributed cell fate determinants and/or distinct cell transcript profiles may be the underlying reason for their distinct fates. However, neither of these hypotheses has convincing support due to technical limitations. Using laser-controlled microdissection, we isolated apical and basal cells and constructed cell type-specific cDNA libraries. Transcript profile analysis revealed difference in transcript composition. PCR and qPCR analysis confirmed that transcripts of selected embryogenesis-related genes were cell-type preferentially distributed. Some of the transcripts that existed in zygotes were found distinctly existed in apical or basal cells. The cell type specific de novo transcription was also found after zygotic cell division. Thus, we found that the transcript diversity occurs between apical and basal cells. Asymmetric zygotic division results in the uneven distribution of some embryogenesis related transcripts in the two-celled proembryos, suggesting that a differential distribution of some specific transcripts in the apical or basal cells may involve in guiding the two cell types to different developmental destinies.
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影响因子：
11.6
作者：
Leonhardt, N;Kwak, JM;Schroeder, JI
通讯作者：
Schroeder, JI
影响因子：
11.6
作者：
Lu, PZ;Porat, R;ONeill, SD
通讯作者：
ONeill, SD
影响因子：
7.2
作者：
He, Yu-Chi;He, Yu-Qing;Yang, Hong-Yuan
通讯作者：
Yang, Hong-Yuan
DOI：
10.1007/s004380050983
发表时间：
1999-03-01
期刊：
MOLECULAR AND GENERAL GENETICS
影响因子：
--
作者：
Dresselhaus, T;Cordts, S;Kranz, E
通讯作者：
Kranz, E
影响因子：
7.2
作者：
De Smet, Ive;Lau, Steffen;Juergens, Gerd
通讯作者：
Juergens, Gerd