Rapid Quantification of Gabapentin, Pregabalin, and Vigabatrin in Human Serum by Ultraperformance Liquid Chromatography With Mass-Spectrometric Detection
Rapid Quantification of Gabapentin, Pregabalin, and Vigabatrin in Human Serum by Ultraperformance Liquid Chromatography With Mass-Spectrometric Detection
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DOI：
10.1097/ftd.0b013e31827788c0
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发表时间：
2013-02-01
影响因子：
2.5
通讯作者：
Swart, Eleanora L.
中科院分区：
文献类型：
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作者：
Chahbouni, Abdel;Sinjewel, Arno;Swart, Eleanora L.
背景：加巴喷丁（GBP）、普瑞巴林（PRG）和氨己烯酸（VIG）用于预防和治疗癫痫发作。所开发的方法被施加到从受试者的GBP.Methods的药代动力学研究的样本：样品预处理包括加入20 μ L的三氯乙酸（30%;体积/体积）和200 μ L的GBP-D4在乙腈中作为内标，以20 μ L的血清。使用Kinetex RP 18柱在Acquity分离模块上进行色谱分离。水相和有机移动的相分别为补充有0.1%甲酸的2 mM乙酸铵水溶液和乙腈。结果：GBP、PRG和VIG的线性范围分别为0.03 ~ 25 mg/L、0.03 ~ 25 mg/L和0.06 ~ 50 mg/L。运行间和运行内准确度范围为90%至107%。该方法的批间和批内不精密度为
Background: Gabapentin (GBP), pregabalin (PRG), and vigabatrin (VIG) are used for the prevention and treatment of epileptic seizures. The developed method was applied to samples from subjects participating in a pharmacokinetic study of GBP.Methods: Sample pretreatment consisted of adding 20 mu L of trichloroacetic acid (30%; vol/vol) and 200 mu L of GBP-d4 in acetonitrile as an internal standard to 20 mu L of serum. Chromatographic separation was performed on an Acquity separation module using a Kinetex RP18 column. The aqueous and organic mobile phases were 2 mM ammonium acetate supplemented with 0.1% formic acid in water and acetonitrile, respectively. The detection by a tandem quadrupole mass spectrometer, operating in the positive mode using multiple reaction monitoring, was completed within 2 minutes.Results: The method was linear over the range of 0.03-25 mg/L for GBP, 0.03-25 mg/L for PRG, and 0.06-50 mg/L for VIG. The between-and within-run accuracies ranged from 90% to 107%. The between-and within-run imprecisions of the method were