Real-world carbon nanoparticle exposures induce brain and gonadal alterations in zebrafish (Danio rerio) as determined by biospectroscopy techniques
Real-world carbon nanoparticle exposures induce brain and gonadal alterations in zebrafish (Danio rerio) as determined by biospectroscopy techniques
复制标题
DOI：
10.1039/c4an02227k
复制
发表时间：
2015-01-01
期刊：
影响因子：
4.2
通讯作者：
Martin, Francis L.
中科院分区：
文献类型：
--
作者：
Li, Junyi;Ying, Guang-Guo;Martin, Francis L.
碳基纳米颗粒（CNPs）是一种新型的人造材料，具有广泛的应用前景，但它可能对生物体构成重大风险。为了弥补纳米毒理学知识的差距，进行了大量的体外或体内研究。然而，毒性数据仍然有限。在此，我们采用了生物光谱学方法来评估CNP诱导的斑马鱼（Danio rerio）的影响。斑马鱼暴露于富勒烯（C60），长或短的多壁碳纳米管（MWCNTs），或单壁碳纳米管（SWCNTs）在两个浓度为21天：0.1毫克L-1或0.001毫克L-1。暴露后，脑，鳃，性腺和肝脏从斑马鱼询问衰减全反射傅里叶变换红外（ATR-FTIR）或拉曼光谱。然后将计算分析应用于所获得的红外（IR）光谱，并且观察到暴露组织与对照之间的明显的生物化学分离，并鉴定出改变的光谱生物标志物。此外，在所有四个组织中的脂质与蛋白质的比例进行了计算的红外光谱，脑和性腺中的不饱和脂质水平进行了评估，通过拉曼光谱。观察到明显的脂质变化。这些研究结果表明，生物光谱学方法有可能检测CNP诱导的斑马鱼生化变化。
Carbon-based nanoparticles (CNPs) have emerged as novel man-made materials with diverse applications, which may present significant risks to organisms. To bridge the gap in our knowledge of nano-toxicology, a number of in vitro or in vivo studies have been carried out. However, toxicity data remains limited. Herein, we employed a biospectroscopy approach to assess CNP-induced effects in zebrafish (Danio rerio). Zebrafish were exposed to Fullerene (C60), long or short multi-walled carbon nanotubes (MWCNTs), or single-walled carbon nanotubes (SWCNTs) for 21 days at two concentrations: 0.1 mg L-1 or 0.001 mg L-1. Following exposure, the brain, gills, gonads and liver from zebrafish were interrogated by attenuated total reflection Fourier-transform infrared (ATR-FTIR) or Raman spectroscopy. Computational analysis was then applied to the acquired infrared (IR) spectra, and distinct biochemical segregations between the exposed tissues vs. control were observed with spectral biomarkers of alterations identified. In addition, lipid-to-protein ratios in all four tissues were calculated by the IR spectra; unsaturated lipid levels in brain and gonad were assessed by Raman spectroscopy. Marked lipid alterations were observed. These findings show that biospectroscopy approaches have the potential to detect CNP-induced biochemical alterations in zebrafish.