The genetic architecture of the MHC class II region in British Texel sheep.
The genetic architecture of the MHC class II region in British Texel sheep.
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DOI：
10.1007/s00251-016-0962-6
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发表时间：
2017-03
期刊：
影响因子：
3.2
通讯作者：
Stear MJ
中科院分区：
文献类型：
--
作者：
Ali AO;Stear A;Fairlie-Clarke K;Brujeni GN;Isa NM;Salisi MS;Donskow-Łysoniewska K;Groth D;Buitkamp J;Stear MJ
了解主要组织相容性复合体的结构，特别是等位基因、基因座和单倍型的数量和频率，对于有效研究MHC影响疾病易感性的方式至关重要。线虫感染是绵羊遭受的最重要的疾病之一，并且II类区域已经反复与对感染的易感性和抵抗力的差异相关联。特克塞尔羊被广泛用于许多不同的国家和相对抵抗感染。本研究确定了一小群特克塞尔羊的MHC II类基因的数量和频率。DRB 1有18个等位基因，DQA 1有9个，DQB 1有13个，DQA 2有8个，DQB 2有16个。一些单倍型在DQA 1、DQB 1或DQB 2处没有可检测的基因产物，这些被定义为无效等位基因。尽管有大量的等位基因，但在种群中只有21种不同的单倍型。观察到的相对较少的单倍型将简化发现疾病关联，因为常见的单倍型提供更多的统计功效，但使从连锁标记基因座区分致病突变复杂化。本文的在线版本（doi：10.1007/s 00251 -016-0962-6）包含补充材料，可供授权用户使用。
Understanding the structure of the major histocompatibility complex, especially the number and frequency of alleles, loci and haplotypes, is crucial for efficient investigation of the way in which the MHC influences susceptibility to disease. Nematode infection is one of the most important diseases suffered by sheep, and the class II region has been repeatedly associated with differences in susceptibility and resistance to infection. Texel sheep are widely used in many different countries and are relatively resistant to infection. This study determined the number and frequency of MHC class II genes in a small flock of Texel sheep. There were 18 alleles at DRB1, 9 alleles at DQA1, 13 alleles at DQB1, 8 alleles at DQA2 and 16 alleles at DQB2. Several haplotypes had no detectable gene products at DQA1, DQB1 or DQB2, and these were defined as null alleles. Despite the large numbers of alleles, there were only 21 distinct haplotypes in the population. The relatively small number of observed haplotypes will simplify finding disease associations because common haplotypes provide more statistical power but complicate the discrimination of causative mutations from linked marker loci. The online version of this article (doi:10.1007/s00251-016-0962-6) contains supplementary material, which is available to authorized users.