Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in north America and Asia
Molecular, serological, and virulence characteristics of Vibrio parahaemolyticus isolated from environmental, food, and clinical sources in north America and Asia
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DOI：
10.1128/aem.69.7.3999-4005.2003
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发表时间：
2003-07-01
影响因子：
4.4
通讯作者：
Gendel, SM
中科院分区：
文献类型：
--
作者：
DePaola, A;Ulaszek, J;Gendel, SM
对来自美国太平洋、大西洋和墨西哥湾沿岸的临床、环境和食物来源以及亚洲临床来源的 178 种致病性副溶血性弧菌分离株的潜在毒力属性、血清型和核糖型进行了测定。食品和环境分离物通常来自牡蛎，通过使用 DNA 探针检测耐热直接溶血素 (tdh) 基因的存在，将其定义为致病性。来自美国的临床分离株通常与牡蛎食用有关，大多数是从华盛顿、德克萨斯和纽约的疫情中获得的。使用多重 PCR 来确认物种鉴定和 tdh 的存在，并测试 tdh 相关的溶血素 trh。来自美国的大多数环境、食品和临床分离株的 tdh、trh 和脲酶生产呈阳性。来自德克萨斯州、纽约州和亚洲的与疫情相关的分离株主要是 O3:K6 血清型，并且仅具有 tdh。分离株中总共鉴定出 27 个血清型和 28 个核糖基，但不同血清型和核糖基之间的菌株分布模式不同。除一株来自德克萨斯州的 O3:K6 分离株外，所有分离株均与来自纽约或亚洲的 O3:K6 分离株处于不同的核糖组中。在来自美国的任何环境和食品分离株中均未检测到 O3:K6 血清型，并且没有任何食品或环境分离株属于包含所有 O3:K6 和相关临床分离株的三个核糖基中的任何一个。血清分型和核糖分型的结合表明，太平洋海岸副溶血弧菌种群似乎与大西洋海岸或墨西哥湾沿岸的种群不同。某些血清型和核糖型包含临床和环境分离株，而许多其他血清型和核糖型仅包含环境分离株，这一事实意味着某些血清型或核糖型与人类疾病更相关。
Potential virulence attributes, serotypes, and ribotypes were determined for 178 pathogenic Vibrio parahaemolyticus isolates from clinical, environmental, and food sources on the Pacific, Atlantic, and Gulf Coasts of the United States and from clinical sources in Asia. The food and environmental isolates were generally from oysters, and they were defined as being pathogenic by using DNA probes to detect the presence of the thermostable direct hemolysin (tdh) gene. The clinical isolates from the United States were generally associated with oyster consumption, and most were obtained from outbreaks in Washington, Texas, and New York. Multiplex PCR was used to confirm the species identification and the presence of tdh and to test for the tdh-related hemolysin trh. Most of the environmental, food, and clinical isolates from the United States were positive for tdh, trh, and urease production. Outbreak-associated isolates from Texas, New York, and Asia were predominantly serotype O3:K6 and possessed only tdh. A total of 27 serotypes and 28 ribogroups were identified among the isolates, but the patterns of strain distribution differed between the serotypes and ribogroups. All but one of the O3:K6 isolates from Texas were in a different ribogroup from the O3:K6 isolates from New York or Asia. The O3:K6 serotype was not detected in any of the environmental and food isolates from the United States, and none of the food or environmental isolates belonged to any of the three ribogroups that contained all of the O3:K6 and related clinical isolates. The combination of serotyping and ribotyping showed that the Pacific Coast V. parahaemolyticus population appeared to be distinct from that of either the Atlantic Coast or Gulf Coast. The fact that certain serotypes and ribotypes contained both clinical and environmental isolates while many others contained only environmental isolates implies that certain serotypes or ribotypes are more relevant for human disease.