FolX from Pseudomonas aeruginosa is octameric in both crystal and solution.
FolX from Pseudomonas aeruginosa is octameric in both crystal and solution.
复制标题
来自铜绿假单胞菌的 FolX 在晶体和溶液中都是八聚体。
DOI：
10.1016/j.febslet.2012.03.031
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发表时间：
2012
期刊：
影响因子：
3.5
通讯作者：
Gabrielsen M
中科院分区：
文献类型：
--
作者：
Gabrielsen M
FolX编码一种差向异构酶，它构成四氢叶酸生物合成途径的一个步骤，这一点很有趣，因为它是重要药物的既定靶点。在这里，我们报告的FolX的晶体结构的细菌机会致病菌铜绿假单胞菌，以及在溶液中的蛋白质的详细分析，使用分析超离心（AUC）和小角X射线散射（SAXS）。结合这些技术，证实了蛋白质在晶体结构和溶液中都是八聚体。蛋白质相互作用的结构总结：FolX和FolX通过X射线晶体学结合（查看相互作用）FolX和FolX通过溶液中的共沉淀结合（查看相互作用）FolX和FolX通过X射线散射结合（查看相互作用）
FolX encodes an epimerase that forms one step of the tetrahydrofolate biosynthetic pathway, which is of interest as it is an established target for important drugs. Here we report the crystal structure of FolX from the bacterial opportunistic pathogen Pseudomonas aeruginosa, as well as a detailed analysis of the protein in solution, using analytical ultracentrifugation (AUC) and small-angle X-ray scattering (SAXS). In combination, these techniques confirm that the protein is an octamer both in the crystal structure, and in solution. STRUCTURED SUMMARY OF PROTEIN INTERACTIONS: FolX and FolXbind by x-ray crystallography (View interaction) FolX and FolXbind by cosedimentation in solution (View interaction) FolX and FolXbind by x ray scattering (View interaction)