Characterization of growth-phase-specific responses to cold in Arabidopsis thaliana suspension-cultured cells.
Characterization of growth-phase-specific responses to cold in Arabidopsis thaliana suspension-cultured cells.
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DOI：
10.1111/j.1365-3040.2007.01767.x
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发表时间：
2008-03
期刊：
影响因子：
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通讯作者：
Yutaka Sasaki;Kazue Takahashi;Yoko Oono;M. Seki;R. Yoshida;K. Shinozaki;M. Uemura
中科院分区：
文献类型：
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作者：
Yutaka Sasaki;Kazue Takahashi;Yoko Oono;M. Seki;R. Yoshida;K. Shinozaki;M. Uemura
为了了解植物细胞对低温的复杂反应，对拟南芥（T87系）悬浮培养细胞在冷处理期间进行了表征。在滞后期或对数期收集的细胞的冷冻耐受性是完全不同的：在冷处理期间冷冻耐受性的增加仅在滞后期的细胞中检测到。虽然在两个生长阶段的细胞渗透压的差异不大，糖含量增加，在冷处理仅在滞后期细胞。脱落酸（阿坝）含量在整个冷处理的滞后期比在对数期更大，增加只在滞后期细胞。有趣的是，阿坝处理只在滞后期细胞中导致抗冻性的增加。冷响应基因如DREB 1A/CBF 3，COR 15 a和RD 29 A的表达发生在两个生长期的细胞中;然而，COR 15 a和RD 29 A的诱导程度在滞后期的细胞中略大于对数期。基因芯片分析表明，冷调节基因（COR基因）可分为3组：冷处理过程中持续上调或下调，冷处理1 d后短暂上调，冷处理2 d后下调。在仅在滞后期细胞中冷处理1d后表达上调的基因中，通常推断出激酶和转录因子等信号转导上游的功能。这些结果共同表明，这些基因似乎与冷冻耐受诱导细胞在滞后期冷处理。
To understand complex responses of plant cells to low temperatures, suspension-cultured cells of Arabidopsis thaliana (line T87) were characterized during cold treatment. Freezing tolerance of cells collected at the lag or log phase was quite different: an increase in freezing tolerance during cold treatment was only detectable with cells at the lag phase. Although there were little differences in the osmolality of cells at the two growth phases, sugar content increased during cold treatment only in lag phase cells. Abscisic acid (ABA) content was greater at the lag phase than at the log phase throughout the cold treatment, and increased only in lag phase cells. Interestingly, ABA treatment resulted in an increase in freezing tolerance only in lag phase cells. Expression of cold-responsive genes such as DREB1A/CBF3, COR15a and RD29A occurred in cells at the two growth phases; however, the extent of the induction of COR15a and RD29A was somewhat greater in cells at the lag phase than at the log phase. Microarray analysis revealed that cold-regulated genes (COR genes) were categorized into three groups: up- or down-regulated consistently during cold treatment, transiently after 1 d and later after 2 d of cold treatment. Among genes that were up-regulated throughout or transiently after 1 d of cold treatment only in lag phase cells, functions in upstream of signal transduction such as kinase and transcription factor were often deduced. These results collectively suggest that these genes seem to be associated with induction of freezing tolerance by cold treatment in cells at the lag phase.