THE SUBSYNAPTIC 43-KDA PROTEIN IS CONCENTRATED AT DEVELOPING NERVE MUSCLE SYNAPSES INVITRO
THE SUBSYNAPTIC 43-KDA PROTEIN IS CONCENTRATED AT DEVELOPING NERVE MUSCLE SYNAPSES INVITRO
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DOI：
10.1073/pnas.82.23.8270
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发表时间：
1985-01-01
影响因子：
11.1
通讯作者：
BURDEN, SJ
中科院分区：
文献类型：
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作者：
BURDEN, SJ
已知一种43-kDa的外周膜蛋白与从加利福尼亚电鳐（Torpedo californica）的电器官分离的富含乙酰胆碱受体（AcChoR）的膜共纯化。免疫电镜和交联研究表明，这种43-kDa蛋白与AcChoR的胞质结构域密切相关，并表明43-kDa蛋白可以调节AcChoR在突触后膜中的分布。本文表明，这种突触后蛋白出现在非洲爪蟾神经/肌肉共培养的神经肌肉突触，早在AcChoRs成为聚集在突触部位。此外，这种蛋白质集中在非神经支配的肌肉细胞上出现的AcChoR簇。这种突触下蛋白和AcChoR簇的密切空间和时间关系与43 kDa蛋白在AcChoR簇的形成和/或稳定中的作用一致。
A 43-kDa peripheral membrane protein is known to copurify with acetylcholine receptor (AcChoR)-rich membranes isolated from the electric organ of Torpedo californica. Immunoelectron microscopy and crosslinking studies have demonstrated that this 43-kDa protein is closely associated with the cytoplasmic domain(s) of the AcChoR and suggest that the 43-kDa protein could regulate the distribution of the AcChoR in the postsynaptic membrane. This paper demonstrates that this postsynaptic protein appears at developing neuromuscular synapses in Xenopus nerve/muscle cocultures as early as AcChoRs become clustered at synaptic sites. Moreover, this protein is concentrated at AcChoR clusters that occur on noninnervated muscle cells. The close spatial and temporal relationship of this subsynaptic protein and AcChoR clusters is consistent with a role for the 43-kDa protein in the formation and/or stabilization of AcChoR clusters.