Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules.
Preparation of thermoresponsive anionic copolymer brush surfaces for separating basic biomolecules.
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DOI：
10.1021/bm9010744
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发表时间：
2010-01
影响因子：
6.2
通讯作者：
K. Nagase;J. Kobayashi;A. Kikuchi;Y. Akiyama;H. Kanazawa;M. Annaka;T. Okano
中科院分区：
文献类型：
--
作者：
K. Nagase;J. Kobayashi;A. Kikuchi;Y. Akiyama;H. Kanazawa;M. Annaka;T. Okano
聚(N-异丙基丙烯酰胺-共-丙烯酸-共-N-叔丁基丙烯酰胺)(聚(IPAAm-共-AAc-共-tBAAm)刷接枝二氧化硅珠是通过表面引发原子转移自由基聚合(ATRP)与CuCl/CuCl(2)/Me(6)TREN催化体系在2-丙醇中在25℃下制备4 h.通过色谱分析对制备的珠进行表征。由于其高密度接枝共聚物结构，碱性分析物、儿茶酚胺衍生物和血管紧张素肽可以通过包含珠子的短柱长度进行分离。由于儿茶酚胺衍生物与密集接枝阴离子的静电和疏水相互作用，获得了具有高分辨率峰的色谱图 珠子上的共聚物。血管紧张素肽的有效分离是在共聚物的较低临界溶液温度附近进行的，因为共聚物和分析物之间的总静电和疏水相互作用在该温度下变强。这些结果表明，ATRP 制备的共聚物刷接枝表面是通过调节静电和疏水相互作用来分离基本生物分子的有效工具。
Poly(N-isopropylacrylamide-co-acrylic acid-co-N-tert-butylacrylamide) (poly(IPAAm-co-AAc-co-tBAAm) brush grafted silica beads were prepared through a surface-initiated atom transfer radical polymerization (ATRP) with CuCl/CuCl(2)/Me(6)TREN catalytic system in 2-propanol at 25 degrees C for 4 h. The prepared beads were characterized by chromatographic analysis. Basic analytes, catecholamine derivatives, and angiotensin peptides could be separated by a short column length containing the beads because of its high densely grafted copolymer structure. Chromatograms for catecholamine derivatives were obtained with high resolution peaks due to their electrostatic and hydrophobic interactions to the densely grafted anionic copolymers on the beads. Effective separation of angiotensin peptides was performed near the lower critical solution temperature of copolymers, because the total electrostatic and hydrophobic interactions between the copolymer and the analytes become strong at the temperature. These results indicated that the copolymer brush grafted surfaces prepared by ATRP was an effective tool for separating basic biomolecules by modulating the electrostatic and hydrophobic interactions.