Ectopic expression of chloramphenicol acetyltransferase (CAT) in the cerebellum in mice transgenic for a carbonic anhydrase II promoter-CAT construct that is without apparent phenotypic effect.
Ectopic expression of chloramphenicol acetyltransferase (CAT) in the cerebellum in mice transgenic for a carbonic anhydrase II promoter-CAT construct that is without apparent phenotypic effect.
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碳酸酐酶 II 启动子-CAT 构建体转基因小鼠小脑中氯霉素乙酰转移酶 (CAT) 的异位表达，但没有明显的表型效应。
DOI：
10.1002/mrd.1080270204
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发表时间：
1990
影响因子：
2.5
通讯作者：
Tashian,RE
中科院分区：
文献类型：
--
作者：
Erickson,RP;Bevilacqua,A;Venta,PJ;Karolyi,J;Tashian,RE
我们已经建立了六个转基因小鼠系，构建了含有假定的碳酸酐酶II(CA II)5‘调控区的结构。其中4个品系含有人CAⅡ基因5‘侧翼区1100个碱基，2个转基因品系由含有500个碱基的小鼠CA II基因5’侧翼区构建而成。氯霉素乙酰转移酶(CAT)基因在所有转基因株系中均未获得组织特异性表达。其中一个转基因品系在小脑中有高水平的CAT表达。这种表达在多个世代中持续存在，并与转基因的亲本来源无关。假设这种表达是由于在小脑中正常表达的基因中或附近插入了转基因，培育了纯合子小鼠以进行转基因插入，以确定是否已经诱导了突变。纯合子小鼠被发现，并似乎是正常的所有方面的表型研究。因此，在这种情况下，无论是基因的插入还是CAT的异位表达似乎都不会对动物造成伤害。
We have developed six transgenic lines of mice with constructs containing presumptive 5′ regulatory regions of carbonic anhydrase II (CA II). Four of the lines contained 1,100 bases of the 5′ flanking region of the human CA II gene, and two transgenic lines resulted from a construct containing 500 bases of the 5′ flanking region of the mouse CA II gene. Tissue‐specific expression of the chloramphenicol acetyltransferase (CAT) gene was not obtained in any of the transgenic lines. One of the transgenic lines was found to have high levels of expression of CAT in cerebellum. This expression persisted through multiple generations and was independent of the parental origin of the transgene. On the assumption that the expression was due to the insertion of the transgene in or near a gene expressed normally in cerebellum, homozygous mice were bred for the transgenic insert to see if a mutation might have been induced. Homozygous mice were found and seemed to be normal in all aspects of their phenotype studied. Thus, in this case, neither the insertion of the gene nor the ectopic expression of CAT seemed to be harmful to the animals.