THE CYTOSKELETAL PROTEIN VINCULIN CONTAINS TRANSFORMATION-SENSITIVE, COVALENTLY BOUND LIPID
THE CYTOSKELETAL PROTEIN VINCULIN CONTAINS TRANSFORMATION-SENSITIVE, COVALENTLY BOUND LIPID
复制标题
DOI：
10.1126/science.3099391
复制
发表时间：
1987-01-23
期刊：
影响因子：
56.9
通讯作者：
BURGER, MM
中科院分区：
文献类型：
--
作者：
BURN, P;BURGER, MM
纽蛋白与许多细胞的细胞骨架相关，被认为是微丝束和质膜之间的可能连接物。这里将表明脂肪酸在体内共价连接至纽蛋白。此外，在感染劳斯肉瘤病毒温度敏感突变体tsNY68的鸡胚成纤维细胞中，纽蛋白在允许温度下的酰化程度低于非允许温度下的三分之一。因此，脂质与纽蛋白的共价结合是转化敏感事件。脂质对纽蛋白的共价修饰可能直接或间接参与其与膜的可逆结合。这种修饰还可能提供一种改变细胞内纽蛋白组织的机制，从而在质膜上锚定或稳定微丝束方面发挥调节作用。
Vinculin, which is associated with the cytoskeleton of many cells, has been suggested as a possible linker between microfilament bundles and the plasma membrane. Here it will be shown that fatty acid is covalently attached to vinculin in vivo. Furthermore, in chicken embryo fibroblasts infected with a temperature-sensitive mutant of Rous sarcoma virus, tsNY68, the acylation of vinculin at the permissive temperature was less than one-third that at the nonpermissive temperature. Thus, the covalent binding of lipid to vinculin is a transformation-sensitive event. The covalent modification of vinculin by lipids could be directly or indirectly involved in its reversible association with membranes. This modification may also provide a mechanism to alter the organization of vinculin within cells and thereby play a regulatory role in anchoring or stabilizing microfilament bundles at plasma membranes.