Thiol-Functionalized Block Copolymer Vesicles.
Thiol-Functionalized Block Copolymer Vesicles.
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DOI：
10.1021/mz300318a
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发表时间：
2012-07
影响因子：
5.8
通讯作者：
J. Rosselgong;A. Blanazs;P. Chambon;Mark Williams;M. Semsarilar;J. Madsen;Giuseppe Battaglia;
中科院分区：
文献类型：
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作者：
J. Rosselgong;A. Blanazs;P. Chambon;Mark Williams;M. Semsarilar;J. Madsen;Giuseppe Battaglia;
硫醇官能化嵌段共聚物囊泡很容易通过 RAFT 介导的聚合诱导自组装 (PISA) 制备。更具体地说，二硫化物官能化的聚（单甲基丙烯酸甘油酯）大分子CTA使用甲基丙烯酸2-羟丙酯进行扩链：不断增长的水不溶性聚（甲基丙烯酸2-羟丙酯）链驱动原位自组装，在浓水溶液中产生二嵌段共聚物囊泡。聚（单甲基丙烯酸甘油酯）稳定剂链中的二硫键使用三丁基膦或三（2-羧乙基）膦原位还原断裂，生成硫醇基团，硫醇基团立即与丙烯酸季铵盐反应以引入阳离子特性，或与罗丹明 B 丙烯酸酯或罗丹明 B 异硫氰酸酯反应以赋予方便的荧光标签。除了这种容易的衍生化之外，这种硫醇功能化的囊泡还可能为药物递送应用提供一些潜力，因为这些纳米物体预计会增强粘膜粘附力。
Thiol-functionalized block copolymer vesicles are readily prepared via RAFT-mediated polymerization-induced self-assembly (PISA). More specifically, a disulfide-functionalized poly(glycerol monomethacrylate) macro-CTA is chain-extended using 2-hydroxypropyl methacrylate): the growing water-insoluble poly(2-hydroxypropyl methacrylate) chains drive in situ self-assembly to produce diblock copolymer vesicles in concentrated aqueous solution. The disulfide bonds in the poly(glycerol monomethacrylate) stabilizer chains are reductively cleaved in situ using either tributyl phosphine or tris(2-carboxyethyl)phosphine to generate thiol groups, which react immediately with either a quaternary acrylate to introduce cationic character or with rhodamine B acrylate or rhodamine B isothiocyanate to confer a convenient fluorescent tag. In addition to such facile derivatization, such thiol-functionalized vesicles may offer some potential for drug delivery applications, because enhanced muco-adhesion is anticipated for these nano-objects.