Preparation of L-phenylalanine-imprinted solid-phase extraction sorbent by Pickering emulsion polymerization and the selective enrichment of L-phenylalanine from human urine
Preparation of L-phenylalanine-imprinted solid-phase extraction sorbent by Pickering emulsion polymerization and the selective enrichment of L-phenylalanine from human urine
复制标题
Pickering乳液聚合制备L-苯丙氨酸印迹固相萃取吸附剂及选择性富集人尿中L-苯丙氨酸
DOI：
10.1002/jssc.201600055
复制
发表时间：
2016-05-01
影响因子：
3.1
通讯作者：
Song, Renyuan
中科院分区：
文献类型：
--
作者：
Li, Ji;Hu, Xiaoling;Song, Renyuan
采用Pickering乳液聚合和离子对虚拟模板印迹相结合的方法合成了一种新型L-苯丙氨酸分子印迹固相萃取吸附剂。与其他聚合方法相比，该方法制备的分子印迹聚合物具有高的比表面积、大的孔径和合适的粒径。对固相萃取的关键参数进行了优化，结果表明，所制备的分子印迹聚合物对L-苯丙氨酸的回收率为98.9%。在优化的条件下，建立了L-苯丙氨酸的分析方法。通过比较分子印迹聚合物和商业反相硅胶的性能，得到的分子印迹聚合物作为固相萃取吸附剂是更合适的，表现出高的精密度（相对标准偏差3.2%，n = 4）和低检测限（60.0 +/- 1.9 nmol.L-1）的L-苯丙氨酸的分离。基于这些结果，Pickering乳液聚合和离子对虚拟模板印迹的组合是有效的，用于制备选择性固相萃取吸附剂，从复杂的生物样品中分离氨基酸和有机酸。
A novel L-phenylalanine molecularly imprinted solid-phase extraction sorbent was synthesized by the combination of Pickering emulsion polymerization and ion-pair dummy template imprinting. Compared to other polymerization methods, the molecularly imprinted polymers thus prepared exhibit a high specific surface, large pore diameter, and appropriate particle size. The key parameters for solid-phase extraction were optimized, and the result indicated that the molecularly imprinted polymer thus prepared exhibits a good recovery of 98.9% for L-phenylalanine. Under the optimized conditions of the procedure, an analytical method for L-phenylalanine was well established. By comparing the performance of the molecularly imprinted polymer and a commercial reverse-phase silica gel, the obtained molecularly imprinted polymer as an solid-phase extraction sorbent is more suitable, exhibiting high precision (relative standard deviation 3.2%, n = 4) and a low limit of detection (60.0 +/- 1.9 nmol.L-1) for the isolation of L-phenylalanine. Based on these results, the combination of the Pickering emulsion polymerization and ion-pair dummy template imprinting is effective for preparing selective solid-phase extraction sorbents for the separation of amino acids and organic acids from complex biological samples.