Persistence of Ebola Virus in Sterilized Wastewater.
Persistence of Ebola Virus in Sterilized Wastewater.
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DOI：
10.1021/acs.estlett.5b00193
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发表时间：
2015-09-08
影响因子：
10.9
通讯作者：
Munster VJ
中科院分区：
文献类型：
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作者：
Bibby K;Fischer RJ;Casson LW;Stachler E;Haas CN;Munster VJ
在2014/2015年埃博拉病毒爆发后，有关适当处理埃博拉病毒污染的液体废物的重大问题仍然存在，包括废水中埃博拉病毒的持续存在。为了解决这些不确定性，我们评估了消毒生活污水中埃博拉病毒的持久性。在第一个试验日内，病毒滴度从初始病毒滴度106 TCID 50 mL-1下降约99%;但是，无法确定这种初始快速下降是由于病毒颗粒聚集还是灭活所致。随后的病毒滴度下降不太迅速，感染性埃博拉病毒颗粒持续了所有8天的测试。灭活常数（k）确定为−1.08（病毒滴度降低90%为2.1天）。由于实验条件，我们认为这些结果是埃博拉病毒在废水中持续存在的上限。废水成分本质上是异质性的，因此，我们警告说，这些结果的解释应在一个整体的评估，包括废水成分，稀释和潜在的接触途径的影响，在废水基础设施。虽然目前还不清楚埃博拉病毒是否可能通过废水传播，但这些数据表明了通过废水接触传染性埃博拉病毒的潜在途径，并强调了在流行病应对中对废水处理采取预防性方法的价值。
In the wake of the ongoing 2014/2015 Ebola virus outbreak, significant questions regarding the appropriate handling of Ebola virus-contaminated liquid waste remain, including the persistence of Ebola virus in wastewater. To address these uncertainties, we evaluated the persistence of Ebola virus spiked in sterilized domestic sewage. The viral titer decreased approximately 99% within the first test day from an initial viral titer of 106 TCID50 mL–1; however, it could not be determined if this initial rapid decrease was due to aggregation or inactivation of the viral particles. The subsequent viral titer decrease was less rapid, and infectious Ebola virus particles persisted for all 8 days of the test. The inactivation constant (k) was determined to be −1.08 (2.1 days for a 90% viral titer decrease). Due to experimental conditions, we believe these results to be an upper bound for Ebola virus persistence in wastewater. Wastewater composition is inherently heterogeneous; subsequently, we caution that interpretation of these results should be made within a holistic assessment, including the effects of wastewater composition, dilution, and potential exposure routes within wastewater infrastructure. While it remains unknown if Ebola virus may be transmitted via wastewater, these data demonstrate a potential exposure route to infectious Ebola virus via wastewater and emphasize the value of a precautionary approach to wastewater handling in an epidemic response.