Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
Construction of an anchoring SSR marker genetic linkage map and detection of a sex-linked region in two dioecious populations of red bayberry
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杨梅两个雌雄异株群体锚定SSR标记遗传连锁图谱的构建及性连锁区域的检测
DOI：
10.1038/s41438-020-0276-6
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发表时间：
2020-04
影响因子：
8.7
通讯作者：
Gao Zhong-Shan
中科院分区：
文献类型：
--
作者：
Wang Yan;Jia Hui-Min;Shen Yu-Tong;Zhao Hai-Bo;Yang Qin-Song;Zhu Chang-Qing;Sun De-Li;Wang Guo-Yun;Zhou Chao-Chao;Jiao Yun;Chai Chun-Yan;Yan Li-Ju;Li Xiong-Wei;Jia Hui-Juan;Gao Zhong-Shan
杨梅（莫雷拉rubra）是我国南方的一种万年青果树，其全基因组序列已被公布。通过添加118个SSR标记和雌性特异标记MrFT 2_BD-SEX，更新了该物种的连锁图谱。该图谱包括8个连锁群，总长度为491 cM。鉴定了11个性别相关标记，其中6个位于连锁群8，与先前报道的性别决定区的位置一致。利用MrFT 2_BD-SEX标记对203份栽培材料进行了基因分型。在供试材料的雌性中，我们发现了两个雌性特有的等位基因，分别命名为W-b（151 bp）和W-d（129 bp）。我们以前发现，‘东葵’，一个雌性品种，可以产生有活力的花粉（我们称这样的植物‘东葵雄性’），并作为父本杂交。MrFT 2_BD-SEX标记的基因型在“碧奇”中为B/Z，在“东魁雄”中为W-d/Z。这些亲本之间杂交的后代产生3：1的雌性（W-）与雄性（ZZ）比率和预期的1：1：1：1的W-b/W-d：W-b/Z：W-d/Z：Z/Z比率。此外，F1代的开花和结实表型均符合其基因型。我们的研究结果证实了ZW性别决定的存在，并表明女性表型是由一个单一的显性位点（W）在一个小的基因组区域（59 kb和小于3.3 cM）。此外，我们还培育出了纯合的“超级雌性”（WW），它应该会在F2代产生全雌性后代，为商业用途提供基础，并在现代育种计划中展现出巨大的应用潜力。
Red bayberry (Morella rubra) is an evergreen fruit tree found in southern China whose whole-genome sequence has recently been published. We updated the linkage map of the species by adding 118 SSR markers and the female-specific marker MrFT2_BD-SEX. The integrated map included eight linkage groups and spanned 491 cM. Eleven sex-associated markers were identified, six of which were located in linkage group 8, in agreement with the previously reported location of the sex-determining region. The MrFT2_BD-SEX marker was genotyped in 203 cultivated accessions. Among the females of the accessions, we found two female-specific alleles, designated W-b (151 bp) and W-d (129 bp). We previously found that 'Dongkui', a female cultivar, could produce viable pollen (we refer to such plants 'Dongkui-male') and serve as the paternal parent in crosses. The genotypes of the MrFT2_BD-SEX marker were W-b/Z in 'Biqi' and W-d/Z in 'Dongkui-male'. The progeny of a cross between these parents produced a 3:1 female (W-) to male (ZZ) ratio and the expected 1:1:1:1 ratio of W-b/W-d: W-b/Z: W-d/Z: Z/Z. In addition, the flowering and fruiting phenotypes of all the F1 progeny fit their genotypes. Our results confirm the existence of ZW sex determination and show that the female phenotype is controlled by a single dominant locus (W) in a small genomic region (59 kb and less than 3.3 cM). Furthermore, we have produced a homozygous "super female" (WW) that should produce all-female offspring in the F2 generation, providing a foundation for commercial use and presenting great potential for use in modern breeding programs.
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影响因子：
3.3
作者：
J. K. Pritchard;Matthew Stephens;Peter Donnelly
通讯作者：
J. K. Pritchard;Matthew Stephens;Peter Donnelly
影响因子：
3.8
作者：
Pucholt, P.;Ronnberg-Wastljung, A-C;Berlin, S.
通讯作者：
Berlin, S.
影响因子：
3.1
作者：
Telgmann-Rauber, Alexa;Jamsari, Ari;Jung, Christian
通讯作者：
Jung, Christian
影响因子：
18
作者：
Akagi, Takashi;Pilkington, Sarah M.;Tao, Ryutaro
通讯作者：
Tao, Ryutaro
影响因子：
10.7
作者：
Hollister, Jesse D.;Ross-Ibarra, Jeffrey;Gaut, Brandon S.
通讯作者：
Gaut, Brandon S.