Increased number of new neurons in the olfactory bulb and hippocampus of adult non-human primates after focal ischemia
Increased number of new neurons in the olfactory bulb and hippocampus of adult non-human primates after focal ischemia
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DOI：
10.1016/j.expneurol.2006.03.012
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发表时间：
2006-05-01
影响因子：
5.3
通讯作者：
Hisatsune, Tatsuhiro
中科院分区：
文献类型：
--
作者：
Koketsu, Daisuke;Furuichi, Yasuhisa;Hisatsune, Tatsuhiro
成人神经发生受生长因子、身体条件和其他物理微环境的改变调节。我们研究了局灶性缺血对成年非人灵长类动物食蟹猴脑室下区（SVZ）、嗅球（OB）和海马齿状回（DG）（已知为持续性神经发生区域）神经发生的影响。三只猴子进行大脑中动脉闭塞引起的局灶性缺血，并在缺血后的前2周内多次注射BrdU。28天后，对动物进行灌注。缺血猴同侧DG中新生神经元的数量（3182 +/- 408/mm 3）是未手术猴DG中新生神经元数量的4.7倍。缺血猴同侧嗅球新生神经元数量（9176 ± 2295个/mm 3）是正常嗅球的18.0倍。这些观察结果表明，在灵长类动物局灶性缺血后，新的OB神经元，以及新的DG神经元的数量增加。局灶性缺血后新神经元的大量增加可能是由于细胞增殖的增强而不是细胞定型速率的变化。在三只遭受缺血的猴子中，只有一只动物在同侧SVZ的最前部具有独特的祖细胞类型。在该区域内，观察到表达doublecortin的未成熟神经元祖细胞的短迁移（类似于500 μ m）。(c)2006年爱思唯尔公司All rights reserved.
Adult neurogenesis is modulated by growth factors, physical conditions, and other alterations in the physical microenvironment. We studied the effects of focal ischemia on neurogenesis in the subventricular zone (SVZ), olfactory bulb (OB), and hippocampal dentate gyrus (DG) (known to be persistent neurogenic regions) in the adult non-human primate, the cynomolgus monkey. Three monkeys underwent middle cerebral artery occlusion-induced focal ischemia and were given multiple BrdU injections during the first 2 weeks after ischemia. Twenty-eight days later, the animals were perfused. The number of new neurons (3182 +/- 408/mm(3)) in the ipsilateral DG of ischemic monkeys was 4.7-fold that in the DG of non-operated monkeys. The number of new neurons (9176 +/- 2295/mm(3)) in the ipsilateral olfactory bulb of ischemic monkeys was 18.0-fold that in normal olfactory bulb. These observations suggest an increase in the number of new OB neurons, as well as new DG neurons, after focal ischemia in a primate. This substantial increase in new neurons after focal ischemia could result from the enhancement of cell proliferation rather than a change in the rate of cell commitment. Of the three monkeys subjected to ischemia, only one animal possessed a unique progenitor cell type at the most anterior aspect of the ipsilateral SVZ. Within this region, a short migration (similar to 500 mu m) of doublecortin-expressing immature neuronal progenitor cells was observed. (c) 2006 Elsevier Inc. All rights reserved.