Evidence that large synaptic vesicles containing substance P and small synaptic vesicles have a surface antigen in common in rat.
Evidence that large synaptic vesicles containing substance P and small synaptic vesicles have a surface antigen in common in rat.
复制标题
有证据表明，大鼠中含有 P 物质的大突触囊泡和小突触囊泡具有共同的表面抗原。
DOI：
10.1016/0304-3940(85)90249-6
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发表时间：
1985
影响因子：
2.5
通讯作者：
Leeman,SE
中科院分区：
文献类型：
--
作者：
Floor,E;Leeman,SE
用渗透裂解和CPG-3000可控孔玻璃珠层析的方法从大鼠脑突触体中纯化突触小泡。W.D.Matthew，L.Tsavaler和L.F.Reichardt(J.Cell Biol.，91(1981)257-269)发现，含有P物质(SP)的大突触小泡可选择性地免疫共沉淀来自脑的小(∼)突触小泡(∼50 nm)。含SP囊泡的沉淀与抗体浓度呈可饱和依赖关系。这些发现构成了直接的免疫化学证据，表明来自大脑的大型多肽能突触小泡和小突触小泡具有共同的表面抗原。
Synaptic vesicles were purified from rat brain synaptosomes by osmotic lysis and chromatography on CPG-3000 controlled-pore glass beads. Large (∼ 100 nm) synaptic vesicles containing the peptide substance P (SP) were shown to be immunoprecipitated by a monoclonal antibody previously shown by W.D. Matthew, L. Tsavaler and L.F. Reichardt (J. Cell Biol., 91 (1981) 257–269) to selectively immunoprecipitate small (∼ 50 nm) synaptic vesicles from brain. Precipitation of SP-containing vesicles showed a saturable dependence on antibody concentration. These findings constitute direct, immunochemical evidence that large, peptidergic synaptic vesicles and small synaptic vesicles from brain have a surface antigen in common.