An in vivo comparison of topical agents on wound repair
An in vivo comparison of topical agents on wound repair
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DOI：
10.1097/00006534-200109010-00011
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发表时间：
2001-09-01
影响因子：
3.6
通讯作者：
Nanney, LB
中科院分区：
文献类型：
--
作者：
Bennett, LL;Rosenblum, RS;Nanney, LB
选择理想的防腐剂或抗菌剂治疗污染的伤口仍然是一个有争议的决定。临床决策通常是根据体外研究和个人偏好做出的。虽然局部溶液被广泛使用，但它们对伤口愈合的体内比较效果在很大程度上是未知的。（磺胺米隆溶液），10%聚维酮和1%游离碘（聚维酮碘），0.25%次氯酸钠（“半强度”Dakin）、3%过氧化氢和0.25%乙酸-与对照组。在创伤后第4天和第7天分析胶原蛋白产生和细菌菌落计数（n = 4）。在测试的各种治疗方式中，上皮再生没有受到显著影响。Sulfamylon和Dakin溶液显著增加新皮厚度（P < 0.05），而过氧化氢和乙酸显著抑制新皮形成（P < 0.001）。除过氧化氢外，所有处理均显著增加成纤维细胞增殖。磺胺米隆和聚维酮显著促进血管生成（p < 0.05）。Sulfamylon被证明在维持无菌环境方面最有效，同时伴随着增加血管生成、成纤维细胞增殖和真皮厚度，与对照组相比。这些数据表明，选择特定的局部治疗可以影响动物模型中伤口修复的各个方面。这些结果表明，在临床环境中局部治疗的选择应仔细定制，以匹配独特的伤口情况和治疗终点。
Selection of the ideal antiseptic or antimicrobial treatment for contaminated wounds remains a controversial decision. Clinical decisions are often made on the basis of in vitro studies and personal preference. Although topical solutions are widely used, their comparative in vivo effects on wound healing are largely unreported.A porcine wound model was used to compare five commonly used topical agents-5% mafenide acetate (Sulfamylon solution), 10% povidone with 1% free iodine (Betadine), 0.25% sodium hypochlorite ("half-strength" Dakin), 3% hydrogen peroxide, and 0.25% acetic acid-with a control group.Reepithelialization, angiogenesis, neodermal regeneration, fibroblast proliferation, collagen production, and bacterial colony counts were analyzed at 4 and 7 days after wounding (n = 4). Reepithelialization was not significantly influenced among the various treatment modalities tested. Sulfamylon and Dakin solutions significantly increased neodermal thickness (P < 0.05), whereas hydrogen peroxide and acetic acid significantly inhibited neodermal formation (p < 0.001). All treatments except hydrogen peroxide significantly increased fibroblast proliferation. Sulfamylon and Betadine significantly enhanced angiogenesis (p < 0.05). Sulfamylon proved most effective in maintaining an aseptic environment while concomitantly increasing angiogenesis, fibroblast proliferation, and dermal thickness compared with control.These data show that selection of a particular topical treatment can affect various aspects of wound repair in an animal model. These results suggest that the selection of topical treatments in the clinical setting should be carefully tailored to match unique wound situations and therapeutic endpoints.