The Effect of Talaromyces marneffei Infection on CD86 Expression in THP-1 Cells.
The Effect of Talaromyces marneffei Infection on CD86 Expression in THP-1 Cells.
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DOI：
10.2147/idr.s297160
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发表时间：
2021
影响因子：
3.9
通讯作者：
Liu DH
中科院分区：
文献类型：
--
作者：
Yang D;Shen LX;Chen RF;Fu Y;Xu HY;Zhang LN;Liu DH
马尔尼菲踝节菌（T. marneffii）是一种破坏性机会性二态性真菌，可引起致命的踝节菌病，但马尔尼菲踝节菌的清除主要依赖于先天免疫反应。探讨马尔尼菲木霉感染后是否能抑制THP-1细胞CD86的表达并探讨其潜在机制。采用Western blot和免疫电镜检测37℃BHI培养基上培养的马尔尼菲木霉CD86的表达情况。采用Western blot、酶联免疫和免疫荧光检测马尔尼菲木霉孵育的巨噬细胞CD86表达的变化。采用酶联免疫法检测共培养体系上清液中CD86的含量。采用免疫组化和免疫电镜检测马尔尼菲毛虫与巨噬细胞孵育后CD86的表达情况。 Western blot和免疫电镜检测37℃单独培养时马尔尼菲木霉不表达CD86，但免疫组化和免疫电镜检测与巨噬细胞共培养时确实表达CD86。通过Western blot、酶联免疫分析和免疫荧光检测发现，感染马尔尼菲毛虫后72 h，巨噬细胞CD86表达显着降低，而72 h上清液中CD86含量较对照组显着升高。 1）马尔尼菲木霉感染后，THP-1上的CD86表达下降，并且随着感染的进展，逐渐出现M1型巨噬细胞极化不足的情况； 2）马尔尼菲木霉可能会吸附或摄取巨噬细胞在与THP-1细胞接触过程中产生的上清液中的CD86，从而导致巨噬细胞中CD86的消耗。
Talaromyces marneffei (T. marneffei) is a destructive opportunistic dimorphic fungal which can cause lethiferous Talaromycosis, but the clearance of T. marneffei mainly depends on the innate immune response. To investigate whether T. marneffei can inhibit the expression of CD86 in THP-1 cells after infection and discuss the potential mechanisms. Western blot and immunoelectron microscopy were used to detect the CD86 expression on T. marneffei cultured on BHI medium at 37°C. Western blot, enzyme-linked immunoassay and immunofluorescence were used to detect the change of CD86 expression on macrophages incubating with T. marneffei. Enzyme-linked immunoassay was used to detect the content of CD86 in supernatant in the co-culture system. Immunohistochemistry and immunoelectron microscopy were used to detect the expression of CD86 on T. marneffei incubating with macrophages. T. marneffei did not express CD86 when cultured separately at 37°C detected by Western blot and immunoelectron microscopy, but it did express CD86 when incubated with macrophages detected by immunohistochemistry and immunoelectron microscopy. The CD86 expression of macrophages significantly decreased at 72 hours when infected with T. marneffei while the content of CD86 in supernatant significantly increased at 72 hours compared with the control group which were detected by Western blot, enzyme-linked immunoassay and immunofluorescence. 1) After T. marneffei infection, CD86 expression on THP-1 decreased, and with the progression of infection, insufficient polarization of M1 macrophages gradually appeared; 2) T. marneffei may adsorb or uptake CD86 in supernatant produced by macrophages during the contact with THP-1 cells, thus leading to the consumption of CD86 in macrophages.