Fructose intake during pregnancy up-regulates the expression of maternal and fetal hepatic sterol regulatory element-binding protein-1c in rats
Fructose intake during pregnancy up-regulates the expression of maternal and fetal hepatic sterol regulatory element-binding protein-1c in rats
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DOI：
10.1007/s12020-012-9815-8
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发表时间：
2013-08-01
期刊：
影响因子：
3.7
通讯作者：
Sato, Shin
中科院分区：
文献类型：
--
作者：
Mukai, Yuuka;Kumazawa, Maya;Sato, Shin
2型糖尿病和肥胖症的发生与果糖摄入过量有关。然而，妊娠期间果糖摄入对母体和胎儿脂质代谢的影响尚不清楚。本研究的目的是探讨母亲在怀孕期间摄入果糖是否会影响胎儿和母亲的肝脏脂质代谢。将妊娠Wistar大鼠随机分为未处理对照组和果糖处理组，果糖处理组在整个妊娠期间通过饮用水接受果糖。在妊娠第20天，测量母体血浆中的葡萄糖和胰岛素浓度。采用实时荧光定量PCR技术检测雌、胎鼠肝脏中固醇调节元件结合蛋白（SREBP）-1c及其靶基因的mRNA表达。在果糖处理组中观察到母体血浆葡萄糖水平显著高于对照组，表明高血糖。此外，果糖治疗组显示出显着更高的表达水平的母亲和胎儿SREBP-1c的mRNA和蛋白质和脂肪酸合成酶的表达显着升高，该组还显示出降低酰基辅酶A氧化酶水平在母体肝脏。因此，我们的研究结果表明，母亲在怀孕期间摄入果糖会导致母亲高血糖，并上调胎儿和母体肝脏SREBP-1c的表达。这可能会导致成年后代的碳水化合物和脂质代谢缺陷。
Excess fructose consumption is associated with the development of type 2 diabetes and obesity. However, the impact of fructose intake on maternal and fetal lipid metabolism during pregnancy is not known. The aim of this study was to examine whether maternal fructose intake during pregnancy would affect fetal and maternal hepatic lipid metabolism. Pregnant Wistar rats were randomly divided into untreated control and fructose-treated groups; the fructose-treated group received fructose via drinking water throughout pregnancy. On gestational day 20, glucose and insulin concentration in the maternal plasma were measured. The mRNA expression of sterol regulatory element-binding protein (SREBP)-1c and its target genes in the liver of dams and fetuses were analyzed by real-time PCR. Significantly higher maternal plasma glucose levels, indicating hyperglycemia, was observed in the fructose-treated group than in the control group. Furthermore, the fructose-treated group showed significantly higher expression levels of both maternal and fetal SREBP-1c mRNA and protein and significantly elevated expression of fatty acid synthase; the group also showed reduced acyl-CoA oxidase levels in the maternal liver. Thus, our results suggest that maternal fructose intake during pregnancy causes maternal hyperglycemia and up-regulates hepatic SREBP-1c expression in both fetuses and dams. This may lead to defects in carbohydrate and lipid metabolism in the adult offspring.