MAMMALIAN SEC23P HOMOLOG IS RESTRICTED TO THE ENDOPLASMIC-RETICULUM TRANSITIONAL CYTOPLASM
MAMMALIAN SEC23P HOMOLOG IS RESTRICTED TO THE ENDOPLASMIC-RETICULUM TRANSITIONAL CYTOPLASM
复制标题
DOI：
10.1073/pnas.88.19.8611
复制
发表时间：
1991-10-01
影响因子：
11.1
通讯作者：
SCHEKMAN, R
中科院分区：
文献类型：
--
作者：
ORCI, L;RAVAZZOLA, M;SCHEKMAN, R
酵母Sec 23蛋白在体内和体外是将蛋白从内质网（ER）转运到高尔基体所必需的。 Sec 23 p哺乳动物同源物（通过抗体交叉反应检测）在外分泌和内分泌胰腺细胞中的超微结构定位显示出过渡性ER池和高尔基体之间的细胞质区的特定分布，在那里它似乎与过渡性ER池的管状突起相关，以及与囊泡和周围细胞质的群体相关。 当ER-高尔基体运输中断的能量毒物，突起和转移囊泡显着减少，但Sec 23 p免疫反应位点仍然在过渡细胞质中不明显拴膜附着。 这种意料之外的组织程度表明，胞质蛋白，如Sec 23 p，可能保留在细胞质的专门领域。 过渡区内的结构可以组织运输囊泡和Sec蛋白的通量，以确保在分泌途径的这一分支中的有效蛋白运输。
The yeast Sec23 protein is required in vivo and in vitro for transport of proteins from the endoplasmic reticulum (ER) to the Golgi apparatus. Ultrastructural localization of the Sec23p mammalian homologue (detected by antibody cross-reaction) in exocrine and endocrine pancreatic cells shows a specific distribution to the cytoplasmic zone between the transitional ER cisternae and Golgi apparatus where it appears associated with the tubular protuberances of the transitional ER cisternae, as well as with a population of vesicles, and surrounding cytoplasm. When ER-Golgi transport is interrupted with an energy poison, protuberances and transfer vesicles markedly decrease but Sec23p immunoreactive sites remain in the transitional cytoplasm not apparently tethered by membrane attachment. This unanticipated degree of organization suggests that cytosolic proteins, such as Sec23p, may be retained in specialized areas of the cytoplasm. A structure within the transitional zone may organize the flux of transport vesicles and Sec proteins so as to ensure efficient protein traffic in this limb of the secretory pathway.