Diacylglycerol Acyltransferase from Rat Liver Microsomes
Diacylglycerol Acyltransferase from Rat Liver Microsomes
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来自大鼠肝微粒体的二酰基甘油酰基转移酶
DOI：
10.1111/j.1432-1033.1977.tb11576.x
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发表时间：
1977
期刊：
影响因子：
5.4
通讯作者：
S. Numa
中科院分区：
文献类型：
--
作者：
K. Hosaka;U. Schiele;S. Numa
1
二酰甘油酰基转移酶是从大鼠肝微粒体中分离出来的，分别与甘油磷酸酯转移酶和1-酰基甘油磷酸酯转移酶分离。用非离子洗涤剂Triton X-100溶解的微粒体经分子筛层析得到的酶制剂，通过蔗糖密度梯度离心法实现分离。
2.
虽然二酰甘油酰基转移酶和1-酰基甘油磷酰胆碱酰基转移酶没有分离，但这两种酰基转移酶在热稳定性和对巯基结合试剂的敏感性方面是有区别的。
3.
对双甘油酰基转移酶制剂的研究表明，该酶具有广泛的酰基供体专一性，有效地利用了饱和的、单烯的、二烯的和四烯的脂肪酰辅酶A硫代酯。
4.
本文介绍了一种简化的甘油二酯转移酶的测定方法。
1
Diacylglycerol acyltransferase was resolved from rat liver microsomes and separated from glycerolphosphate acyltransferase and 1-acylglycerolphosphate acyltransferase. The separation was achieved by sucrose-density-gradient centrifugation of the enzyme preparation which was obtained by molecular-sieve chromatography of microsomes solubilized with a nonionic detergent, Triton X-100.
2
Although diacylglycerol acyltransferase and 1-acylglycerolphosphorylcholine acyltransferase were not separated from each other, the two acyltransferases were distinguishable with respect to heat stability and sensitivity to sulfhydryl-binding reagents.
3
Studies with the diacylglycerol acyltransferase preparation obtained have shown that this enzyme possesses a broad acyl-donor specificity, utilizing saturated, monoenoic, dienoic and tetraenoic fatty acyl-CoA thioesters efficiently.
4
A simplified assay method for diacylglycerol acyltransferase is described.