Detection ofSingle CellActivity byUsingFluorescence-Based Multiscale pHandOxygenSensors
Detection ofSingle CellActivity byUsingFluorescence-Based Multiscale pHandOxygenSensors
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使用基于荧光的多尺度 pH 和氧传感器检测单细胞活动
DOI：
10.1002/9780470061565.hbb097
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发表时间：
2008
影响因子：
8.4
通讯作者：
Satoshi KatoandYasunori Iribe
中科院分区：
文献类型：
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作者：
Masayasu Suzuki;Takeshi Yamada;Satoshi KatoandYasunori Iribe
在本章中，我们描述了一种高分辨率表面等离子体共振（SPR）成像系统结合一种新型的微孔阵列芯片。我们开发的微孔阵列芯片由金属（Au、Ag或Al）薄膜涂层玻璃基板（SF6）上的高度集成微孔组成。孔大小设计为单个B细胞的大小。当使用银膜和短波长光时，获得最大的亮度梯度。在微阵列中检测小鼠免疫球蛋白G（IgG）。该结果显示了在微孔阵列中真实的实时监测B淋巴细胞产生抗体的可能性。
In this chapter, we describe a high‐resolution surface plasmon resonance (SPR) imaging system combined with a novel microwell array chip. Our developed microwell array chip consists of highly integrated microwells on a metal (Au, Ag, or Al) film–coated glass substrate (SF6). Well size was designed to the size of a single B cell. When silver film and short‐wavelength light were used, the largest brightness gradient was obtained. Mouse immunoglobulin G (IgG) was detected in the microarray. This result shows the possibility of real‐time monitoring for antibody production by B lymphocytes in microwell arrays.