GABPα regulates Oct-3/4 expression in mouse embryonic stem cells
GABPα regulates Oct-3/4 expression in mouse embryonic stem cells
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DOI：
10.1016/j.bbrc.2006.12.071
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发表时间：
2007-02-16
影响因子：
3.1
通讯作者：
Yokota, Takashi
中科院分区：
文献类型：
--
作者：
Kinoshita, Keita;Ura, Hiroki;Yokota, Takashi
Embryonic stem (ES) cells are pluripotent cells derived from the inner cell mass of blastocysts, and transcription factors Oct-3/4, Nanog, Sox2, and STAT3, are essential for their self-renewal. In this study, we searched for molecules downstream of STAT3 in ES cells. Using DNA chip analysis, we obtained GA-repeat binding protein (GABP) alpha. Expression of GABP alpha was restricted to undifferentiated ES cells and controlled by STAT3. We found that the expression level of Oct-3/4 is reduced by knockdown of GABP alpha. On the other hand, GABP alpha-overexpressing ES cells maintained the expression level of Oct-3/4 even in the absence of LIF. Moreover, the induction of Oct-3/4 repressors Cdx-2, Coup-tf1, and GCNF was stimulated by GABP alpha knockdown. These data suggest that GABP alpha upregulates the expression of Oct-3/4 via downregulation of Oct-3/4 repressors. (c) 2006 Elsevier Inc. All rights reserved.
