HNA and ANA high-affinity arrays for detections of DNA and RNA single-base mismatches
HNA and ANA high-affinity arrays for detections of DNA and RNA single-base mismatches
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DOI：
10.1016/j.bios.2008.01.033
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发表时间：
2008-06-15
影响因子：
12.6
通讯作者：
Herdewijn, Piet
中科院分区：
文献类型：
--
作者：
Abramov, Mikhail;Schepers, Guy;Herdewijn, Piet
DNA微阵列和传感器已成为序列信息功能分析的重要工具。最近，我们报道了含有脱氢己醇糖基的嵌合己醇(HNA)和阿三醇(ANA)核苷酸单体很容易获得，并证明了它们的化学结构与DNA和RNA的合成是相容的。在这次交流中，我们描述了一种基于海航和ANA单元的新型分析平台，用于在玻璃固体载体上合成寡核苷酸阵列，用于DNA和RNA靶标的匹配/失配检测。阵列是通过将二烯修饰的寡核苷酸固定在马来酰亚胺活化的玻片上而制备的。为了验证HNA/ANA阵列的选择性和灵敏度，并与常规DNA阵列进行比较，我们选择了HIV-1的逆转录酶基因(密码子74)和蛋白酶基因(密码子10)的序列。两者的信号和匹配/不匹配辨别的相对强度对于应用HNA或ANA阵列(ANA>；HNA&>DNA)的DNA靶标增加了5倍，对于RNA靶标增加了3-3.5倍(ANA&>；HNA&>DNA)。当然，在miRNA检测的新领域，ANA阵列可能被证明是非常有益的，它们的性质应该被更详细地研究。(C)2008爱思唯尔B.V.保留所有权利。
DNA microarrays and sensors have become essential tools in the functional analysis of sequence information. Recently we reported that chimeric hexitol (HNA) and altritol (ANA) nucleotide monomers with an anhydrohexitol sugar moiety are easily available and proved their chemistry to be compatible with DNA and RNA synthesis. In this communication we describe a novel analytical platform based on HNA and ANA units to be used as synthetic oligonucleotide arrays on a glass solid support for match/mismatch detection of DNA and RNA targets. Arrays were fabricated by immobilization of diene-modified oligonucleotides on maleimido-activated glass slides. To demonstrate the selectivity and sensitivity of the HNA/ANA arrays and to compare their properties with regular DNA arrays, sequences in the reverse transcriptase gene (codon 74) and the protease gene of HIV-1 (codon 10) were selected. Both, the relative intensity of the signal and match/mismatch discrimination increased up to fivefold for DNA targets and up to 3-3.5-fold for RNA targets applying HNA or ANA arrays (ANA > HNA > DNA). Certainly in the new field of miRNA detection, ANA arrays could prove very beneficial and their properties should be investigated in more detail. (c) 2008 Elsevier B.V. All rights reserved.