Germination of Bacillus anthracis spores within alveolar macrophages
Germination of Bacillus anthracis spores within alveolar macrophages
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DOI：
10.1046/j.1365-2958.1999.01137.x
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发表时间：
1999-01-01
影响因子：
3.6
通讯作者：
Mock, M
中科院分区：
文献类型：
--
作者：
Guidi-Rontani, C;Weber-Levy, M;Mock, M
吸入炭疽芽孢杆菌孢子引起的感染的致命性是由涉及细菌毒素合成的复杂致病循环引起的。我们用免疫荧光染色、激光共聚焦扫描显微镜和图像细胞仪分析表明肺泡巨噬细胞是B的主要部位。小鼠吸入感染模型中的炭疽菌萌发。炭疽杆菌在鼠巨噬细胞样RAW264.7细胞和鼠肺泡巨噬细胞内萌发。萌发发生在来自吞噬体室的囊泡中。我们还证明了毒素基因及其反式激活因子AtxA在萌发后的巨噬细胞内表达。
The fatal character of the infection caused by inhalation of Bacillus anthracis spores results from a complex pathogenic cycle involving the synthesis of toxins by the bacterium. We have shown using immunofluorescent staining, confocal scanning laser microscopy and image cytometry analysis that the alveolar macrophage was the primary site of B. anthracis germination in a murine inhalation infection model. Bacillus anthracis germinated inside murine macrophage-like RAW264.7 cells and murine alveolar macrophages. Germination occurred in vesicles derived from the phagosomal compartment. We have also demonstrated that the toxin genes and their trans-activator, AtxA, were expressed within the macrophages after germination.