PRIMITIVE-STREAK ORIGIN OF THE CARDIOVASCULAR-SYSTEM IN AVIAN EMBRYOS
PRIMITIVE-STREAK ORIGIN OF THE CARDIOVASCULAR-SYSTEM IN AVIAN EMBRYOS
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DOI：
10.1006/dbio.1993.1276
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发表时间：
1993-10-01
影响因子：
2.7
通讯作者：
SCHOENWOLF, GC
中科院分区：
文献类型：
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作者：
GARCIAMARTINEZ, V;SCHOENWOLF, GC
通过构建鹌鹑/鸡移植嵌合体，使用QH-1（一种古老的内皮细胞/内皮细胞标记物），以及向原始条纹中注射活体荧光染料（DiI），已经绘制了原始条纹中鸟类心血管系统的起源。我们的研究表明，未来的心脏，包括其内膜和心肌层和相邻的壁心包，占据了大部分的吻侧一半的原条在早期连续条纹原肠胚形成阶段。心脏起源于原始条纹，大致为吻尾顺序（即，在条纹中，预期的心球比预期的心室更向嘴侧出现，而预期的心室又比预期的静脉窦更向嘴侧出现），并且心脏的所有层在其每个喙尾亚部处都一致地起源于原始条纹的同一水平。此外，在原肠胚和神经胚阶段的原始条纹的所有rostrocaudal水平含有预期的内皮细胞。同样，这些细胞在条纹内是吻尾排列的，因此头部血管（动脉和静脉）比躯干血管出现在更多的吻条纹水平。心源性细胞的侵入在中期条纹阶段完成，并且条纹内以前由预期的心源性细胞占据的位置被侵入的预期的体节细胞占据。对未来的心源性和内皮细胞在通过原始条纹侵入期间的承诺状态的进一步研究正在进行中。
The origin of the avian cardiovascular system from the primitive streak has been mapped by the construction of quail/chick transplantation chimeras, the use of QH-1 (an antiquail endothelial/endocardial cell marker), and injections of a vital fluorescent dye (DiI) into the primitive streak. Our studies reveal that the prospective heart, including its endocardial and myocardial layers and the adjacent parietal pericardium, occupies much of the rostral half of the primitive streak at early primitive-streak stages of gastrulation. The heart originates from the primitive streak in roughly rostrocaudal sequence (i.e., the prospective bulbus cordis arises more rostrally in the streak than does the prospective ventricle, which in turn arises more rostrally than does the prospective sinus venosus), and all layers of the heart at each of its rostrocaudal subdivisions originate in concert from the same level of the primitive streak. Moreover, all rostrocaudal levels of the primitive streak at gastrula and neurula stages contain prospective endothelial cells. Again, these cells are rostrocaudally ordered within the streak, such that head blood vessels (both arteries and veins) arise at more rostral streak levels than do trunk blood vessels. Ingression of cardiogenic cells is complete by midprimitive-streak stages, and the position formerly occupied by prospective cardiogenic cells within the streak becomes occupied by ingressing prospective somitic cells. Additional studies on the state of commitment of prospective cardiogenic and endothelial cells during their ingression through the primitive streak are underway.