Methanogenic archaea diversity in hyporheic sediments of a small lowland stream
Methanogenic archaea diversity in hyporheic sediments of a small lowland stream
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DOI：
10.1016/j.anaerobe.2014.11.009
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发表时间：
2015-04-01
期刊：
影响因子：
2.3
通讯作者：
Rulik, Martin
中科院分区：
文献类型：
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作者：
Brablcova, Lenka;Buriankova, Iva;Rulik, Martin
对捷克锡特卡河沿岸沿着5个地点产甲烷古菌的丰度和多样性进行了研究。采用冻芯法从0-25 cm和25-50 cm两个深度采集了浅流沉积物样品。采用荧光原位杂交（FISH）、变性梯度凝胶电泳（DGGE）和测序方法对甲烷菌群落进行了分析。产甲烷菌与DAPI染色细胞的比例在所有地点和深度之间变化，平均值为2.08 × 10（5）/g干沉积物，范围为0.37至4.96 × 10（5）细胞/g干沉积物。在DGGE凝胶上的19个不同位点共检测到73条条带，下游位点的产甲烷菌多样性最高。产甲烷菌多样性与沉积物深度之间没有相关性。DGGE图像聚类分析显示，由不同的DGGE条带的数量和相似性的地方组成的三个主要集群。对代表性DGGE条带的测序分析揭示了隶属于甲烷微生物目、甲烷微生物目和甲烷细胞目的成员的微生物型。了解淡水生态系统中产甲烷古菌的分布和多样性对研究河流沉积物中甲烷的动态变化具有重要意义，也有助于理解全球变暖过程。(C)2014爱思唯尔有限公司版权所有。
Abundance and diversity of methanogenic archaea were studied at five localities along a longitudinal profile of a Sitka stream (Czech Republic). Samples of hyporheic sediments were collected from two sediment depths (0-25 cm and 25-50 cm) by freeze-core method. Methanogen community was analyzed by fluorescence in situ hybridization (FISH), denaturing gradient gel electrophoresis (DGGE) and sequencing method. The proportion of methanogens to the DAPI-stained cells varied among all localities and depths with an average value 2.08 x 10(5) per g of dry sediment with the range from 0.37 to 4.96 x 10(5) cells per g of dry sediment. A total of 73 bands were detected at 19 different positions on the DGGE gel and the highest methanogen diversity was found at the downstream located sites. There was no relationship between methanogen diversity and sediment depth. Cluster analysis of DGGE image showed three main clusters consisting of localities that differed in the number and similarity of the DGGE bands. Sequencing analysis of representative DGGE bands revealed phylotypes affiliated with members belonging to the orders Methanosarcinales, Methanomicrobiales and Methanocellales. The knowledge about occurrence and diversity of methanogenic archaea in freshwater ecosystems are essential for methane dynamics in river sediments and can contribute to the understanding of global warming process. (C) 2014 Elsevier Ltd. All rights reserved.