Measurement of fracture callus material properties via nanoindentation
Measurement of fracture callus material properties via nanoindentation
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DOI：
10.1016/j.actbio.2008.02.030
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发表时间：
2008-09-01
影响因子：
9.7
通讯作者：
Morgan, E. F.
中科院分区：
文献类型：
--
作者：
Leong, P. L.;Morgan, E. F.
在骨折愈合中，受伤骨骼恢复稳定性和强度的程度取决于愈合过程中形成的组织的机械性能。虽然许多技术已被用来量化骨折愈伤组织的整体机械行为，但关于单个愈伤组织的材料特性的数据却很少。这项研究的总体目标是量化这些材料特性。骨折后35天，在大鼠骨折愈伤组织的薄（200μm）纵向切片的多个位置进行纳米压痕。压痕后，切片用茜素红 S 和阿新蓝染色，分别获得组织矿物质含量和蛋白多糖含量的半定量估计值。整个愈伤组织的压痕模量变化了三个数量级（0.61-1010 MPa）。这种变化很大程度上是由于多种组织类型的存在：肉芽组织、软骨样组织和编织骨的压痕模量范围为0.61-1.27 MPa（中位数= 0.99 MPa）、1.39-4.42 MPa（中位数= 2.89 MPa）和26.92-1010.00 MPa（中位数= 132.00）兆帕），分别。在茜素红染色区域，压痕模量与染色强度相关（r = 0.62，P = 0.04），表明模量与编织骨中矿物质含量之间呈正相关。此外，沿着皮质骨膜面的编织骨的压痕模量随着距骨折间隙的距离而增加（P = 0.004）。这些结果证明了纳米压痕在表征骨折愈伤组织中存在的异质组织混合物的弹性特性方面的有用性。 (C) 2008 Acta Materialia Inc. 由 Elsevier Ltd 出版。保留所有权利。
In bone fracture healing, the extent to which the injured bone regains stability and strength depends on the mechanical properties of the tissues that are formed during healing. While many techniques have been used to quantify the overall mechanical behavior of fracture calluses, few data exist on the material properties of individual callus tissues. The overall goal of this study was to quantify these material properties. Nanoindentation was performed at multiple locations across thin (200 mu m), longitudinal sections of rat fracture callus at 35 days post fracture. Following indentation, sections were stained with alizarin red S and alcian blue to obtain semi-quantitative estimates of tissue mineral content and proteoglycan content, respectively. Indentation moduli varied over three orders of magnitude (0.61-1010 MPa) throughout the callus. Much of this variation was due to the presence of multiple tissue types: the indentation moduli of granulation tissue, chondroid tissue and woven bone ranged 0.61-1.27 MPa (median = 0.99 MPa), 1.39-4.42 MPa (median = 2.89 MPa) and 26.92-1010.00 MPa (median = 132.00 MPa), respectively. In regions of alizarin red staining, the indentation modulus was correlated (r = 0.62, P = 0.04) with stain intensity, suggesting a positive correlation between modulus and mineral content in woven bone. In addition, the indentation modulus of woven bone along the periosteal aspect of the cortex increased with distance from the fracture gap (P = 0.004). These results demonstrate the usefulness of nanoindentation in characterizing the elastic properties of the heterogeneous mixture of tissues present in bone fracture callus. (C) 2008 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.