Integration of complex larval chemosensory organs into the adult nervous system of Drosophila
Integration of complex larval chemosensory organs into the adult nervous system of Drosophila
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DOI：
10.1242/dev.00879
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发表时间：
2004-01-01
期刊：
影响因子：
4.6
通讯作者：
Stocker, RF
中科院分区：
文献类型：
--
作者：
Gendre, N;Lüer, K;Stocker, RF
果蝇成虫和全变态昆虫的感觉器官基本上来自成虫的盘，因此是成虫特有的。然而，这里提出的实验证据表明，位于沿着咽的三个主要味觉器官的发育设计不同。在一个全面的细胞分析，我们表明，posteriormost的三个器官直接来自一个类似的幼虫器官和其他两个器官出现分裂的第二个幼虫器官。有趣的是，这两个幼虫的器官持续存在，尽管广泛的重组咽。因此，这三个成体器官的大部分神经元都是存活的幼虫神经元。然而，前部器官包括一些在蛹阶段产生的感器。此外，我们观察到第三幼虫咽器官的细胞凋亡。因此，我们的实验数据首次显示了复杂的，完全分化的幼虫感觉器官整合到成年苍蝇的神经系统中，并证明了它们的神经元的胚胎起源。此外，他们认为这种感觉系统的变态是一个复杂的过程，涉及神经元的持久性，产生额外的神经元和神经元死亡。我们的结论是基于对来自P[GAL4]驱动系的报告基因表达、向胚盘期胚胎中注射辣根过氧化物酶、通过胚胎中热休克诱导的翻转进行细胞标记、溴脱氧尿苷出生日期测定和程序性细胞死亡染色的综合分析。它们挑战了感觉器官在变态过程中被替换的普遍观点。
The sense organs of adult Drosophila, and holometabolous insects in general, derive essentially from imaginal discs and hence are adult specific. Experimental evidence presented here, however, suggests a different developmental design for the three largely gustatory sense organs located along the pharynx. In a comprehensive cellular analysis, we show that the posteriormost of the three organs derives directly from a similar larval organ and that the two other organs arise by splitting of a second larval organ. Interestingly, these two larval organs persist despite extensive reorganization of the pharynx. Thus, most of the neurons of the three adult organs are surviving larval neurons. However, the anterior organ includes some sensilla that are generated during pupal stages. Also, we observe apoptosis in a third larval pharyngeal organ. Hence, our experimental data show for the first time the integration of complex, fully differentiated larval sense organs into the nervous system of the adult fly and demonstrate the embryonic origin of their neurons. Moreover, they identify metamorphosis of this sensory system as a complex process involving neuronal persistence, generation of additional neurons and neuronal death. Our conclusions are based on combined analysis of reporter expression from P[GAL4] driver lines, horseradish peroxidase injections into blastoderm stage embryos, cell labeling via heat-shock-induced flip-out in the embryo, bromodeoxyuridine birth dating and staining for programmed cell death. They challenge the general view that sense organs are replaced during metamorphosis.