Epidermal growth factor modulates cholera toxin induced mammary gland development.
Epidermal growth factor modulates cholera toxin induced mammary gland development.
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表皮生长因子调节霍乱毒素诱导的乳腺发育。
DOI：
10.1080/07435809309026681
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发表时间：
1993
影响因子：
2.1
通讯作者：
Kotolski,LC
中科院分区：
文献类型：
--
作者：
Sheffield,LG;Kotolski,LC
卵巢切除的小鼠进行假手术或唾液腺切除，每天注射生理盐水、雌二醇+孕酮、霍乱毒素或雌二醇+孕酮+霍乱毒素，持续18天。乳腺发育评分和DNA增加雌二醇+孕酮，但不是单独的霍乱毒素。与雌二醇+孕酮组合，霍乱毒素增加乳腺发育评分和乳腺DNA。唾液腺切除术降低了雌二醇、孕酮和霍乱毒素诱导乳腺发育的能力。在其他实验中，小鼠用雌二醇+孕酮致敏10天，并取出乳腺组织用于与胰岛素、醛固酮、霍乱毒素和表皮生长因子的各种组合进行体外培养。霍乱毒素与胰岛素和醛固酮联合使用，可促进体外乳腺发育。涎腺切除术降低了霍乱毒素诱导体外乳腺发育的能力。在培养液中加入表皮生长因子可减轻涎腺切除对乳腺发育的影响。生化研究表明，涎腺切除术降低了雌激素和孕激素诱导乳腺组织中环腺苷酸依赖性蛋白激酶水平的能力，也降低了霍乱毒素诱导组织中环腺苷酸积累的能力。这些影响的涎腺切除术被逆转的EGF添加到培养基。
Ovariectomized mice were either sham operated or sialoadenectomized and injected daily for 18 days with saline, estradiol + progesterone, cholera toxin or estradiol + progesterone + cholera toxin. Mammary development score and DNA were increased by estradiol + progesterone, but not by cholera toxin alone. In combination with estradiol + progesterone, cholera toxin increased mammary development score and mammary DNA. Sialoadenectomy reduced the ability of estradiol, progesterone and cholera toxin to induce mammary development. In other experiments, mice were primed with estradiol + progesterone for 10 days, and mammary tissue removed for in vitro culture with various combinations of insulin, aldosterone, cholera toxin and epidermal growth factor. In combination with insulin and aldosterone, cholera toxin increased mammary development in vitro. Sialoadenectomy reduced the ability of cholera toxin to induce mammary development in vitro. The effect of sialoadenectomy on mammary development was alleviated by adding epidermal growth factor to culture medium. Biochemical studies indicated that sialoadenectomy reduced the ability of estrogen and progesterone to induce cyclic AMP dependent protein kinase levels in mammary tissue, and also the ability of cholera toxin to induce accumulation of cyclic AMP in tissues. These effects of sialoadenectomy were reversed by addition of EGF to culture media.