A quantitative x-ray detection system for gold nanoparticle tumour biomarkers
A quantitative x-ray detection system for gold nanoparticle tumour biomarkers
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DOI：
10.1088/0031-9155/57/17/5543
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发表时间：
2012-09-07
影响因子：
3.5
通讯作者：
Royle, G. J.
中科院分区：
文献类型：
--
作者：
Ricketts, K.;Castoldi, A.;Royle, G. J.
X射线荧光技术已被证明有益于识别和量化生物组织中的微量元素。目前正在开发一种新的方法，该方法采用X射线荧光，旨在定位嵌入组织中的重纳米颗粒，如金。这种纳米颗粒可以被功能化，作为肿瘤特征的标记物，以绘制疾病状态，未来的目标是对它们进行成像，以告知癌症治疗方案。癌细胞对功能化纳米颗粒的摄取也将使得能够检测目前被常用的成像方式遗漏的小簇浸润癌细胞。该新系统由具有高光谱分辨率的能量分辨硅漂移探测器组成，在肿瘤中通常发现的纳米颗粒浓度的定量和灵敏度方面都显示出潜力。一系列的同步辐射测量，荧光强度和金纳米粒子（GNP）浓度之间的线性关系被发现下降到0.005 mgAu ml（-1），系统的检测限。成功使用的台式源，适用于未来可能的临床使用，也证明，并发现不降低检测限或GNP浓度测量的准确度。所实现的系统灵敏度表明，在测量体内肿瘤摄取方面，特别是在浅肿瘤部位和小动物中，在离体组织和3D体外研究样品中，未来可能具有临床实用性。
X-ray fluorescence techniques have proven beneficial for identifying and quantifying trace elements in biological tissues. A novel approach is being developed that employs x-ray fluorescence with an aim to locate heavy nanoparticles, such as gold, which are embedded into tissues. Such nanoparticles can be functionalized to act as markers for tumour characteristics to map the disease state, with the future aim of imaging them to inform cancer therapy regimes. The uptake of functionalized nanoparticles by cancer cells will also enable detection of small clusters of infiltrating cancer cells which are currently missed by commonly used imaging modalities. The novel system, consisting of an energy-resolving silicon drift detector with high spectral resolution, shows potential in both quantification of and sensitivity to nanoparticle concentrations typically found in tumours. A series of synchrotron measurements are presented; a linear relationship between fluorescence intensity and gold nanoparticle (GNP) concentration was found down to 0.005 mgAu ml(-1), the detection limit of the system. Successful use of a bench-top source, suitable for possible future clinical use, is also demonstrated, and found not to degrade the detection limit or accuracy of the GNP concentration measurement. The achieved system sensitivity suggests possible future clinical usefulness in measuring tumour uptake in vivo, particularly in shallow tumour sites and small animals, in ex vivo tissue and in 3D in vitro research samples.