Novel Wolbachia strains in Anopheles malaria vectors from sub-Saharan Africa.
Novel Wolbachia strains in Anopheles malaria vectors from sub-Saharan Africa.
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DOI：
10.12688/wellcomeopenres.14765.2
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发表时间：
2018-01-01
影响因子：
--
通讯作者：
Walker, T.
中科院分区：
文献类型：
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作者：
Jeffries, C. L.;Lawrence, G. G.;Walker, T.
背景：沃尔巴克氏体是一种常见的昆虫内共生细菌，可以影响病原体传播并操纵宿主繁殖，历史上一直认为按蚊属中不存在沃尔巴克氏体，但最近在按蚊属中发现了沃尔巴克氏体。冈比亚有限公司西非的人口。由于有许多按蚊物种具有传播疟疾的能力，我们分析了五个疟疾流行国家的一系列物种，以确定沃尔巴克氏体的患病率，表征新的沃尔巴克氏体菌株，并确定疟原虫、沃尔巴克氏体和竞争细菌 Asaia 的存在之间的任何相关性。方法：2013 年至 2017 年间，从五个疟疾流行国家：几内亚、刚果民主共和国 (DRC)、加纳、乌干达和马达加斯加收集按蚊成蚊。使用定量 PCR、桑格测序、沃尔巴克氏菌多位点序列分型 (MLST) 和细菌 16S rRNA 基因的高通量扩增子测序进行分子分析。结果：在五个物种中发现了新的沃尔巴克氏体菌株：按蚊。科鲁兹，An.冈比亚 s.s., An.阿拉伯树，An.穆切蒂和安. A 种，增加已知自然感染的按蚊种类数量。观察到不同地点的不同流行率，新菌株在系统发育上具有多样性，与沃尔巴克氏体 B 超群菌株聚类。我们还提供了 An. 内常驻菌株变异的证据。沃尔巴克氏菌 (A. Wolbachia) 是安氏菌微生物组的主要成员。穆切蒂和安.物种 A，但在 An 中密度较低。科鲁兹。有趣的是，没有发现沃尔巴克氏体/Asaia 共同感染的证据，并且 Asaia 感染密度是可变的且依赖于位置。结论：按蚊中新型沃尔巴克氏体菌株的重要发现，为了解不同疟疾病媒中常驻沃尔巴克氏体菌株的流行情况提供了更深入的了解。新型沃尔巴克氏体菌株（特别是高密度菌株）是转染的理想候选菌株，可在其他按蚊物种中产生稳定的感染，可用于种群替代或抑制控制策略。
Background: Wolbachia, a common insect endosymbiotic bacterium that can influence pathogen transmission and manipulate host reproduction, has historically been considered absent from the Anopheles (An.) genera, but has recently been found in An. gambiae s.l. populations in West Africa. As there are numerous Anopheles species that have the capacity to transmit malaria, we analysed a range of species across five malaria endemic countries to determine Wolbachia prevalence rates, characterise novel Wolbachia strains and determine any correlation between the presence of Plasmodium, Wolbachia and the competing bacterium Asaia. Methods: Anopheles adult mosquitoes were collected from five malaria-endemic countries: Guinea, Democratic Republic of the Congo (DRC), Ghana, Uganda and Madagascar, between 2013 and 2017. Molecular analysis was undertaken using quantitative PCR, Sanger sequencing, Wolbachia multilocus sequence typing (MLST) and high-throughput amplicon sequencing of the bacterial 16S rRNA gene. Results: Novel Wolbachia strains were discovered in five species: An. coluzzii, An. gambiae s.s., An. arabiensis, An. moucheti and An. species A, increasing the number of Anopheles species known to be naturally infected. Variable prevalence rates in different locations were observed and novel strains were phylogenetically diverse, clustering with Wolbachia supergroup B strains. We also provide evidence for resident strain variants within An. species A. Wolbachia is the dominant member of the microbiome in An. moucheti and An. species A but present at lower densities in An. coluzzii. Interestingly, no evidence of Wolbachia/Asaia co-infections was seen and Asaia infection densities were shown to be variable and location dependent. Conclusions: The important discovery of novel Wolbachia strains in Anopheles provides greater insight into the prevalence of resident Wolbachia strains in diverse malaria vectors. Novel Wolbachia strains (particularly high-density strains) are ideal candidate strains for transinfection to create stable infections in other Anopheles mosquito species, which could be used for population replacement or suppression control strategies.