Resveratrol delivery by ultrasound-mediated nanobubbles targeting nucleus pulposus cells
Resveratrol delivery by ultrasound-mediated nanobubbles targeting nucleus pulposus cells
复制标题
通过超声介导的纳米气泡靶向髓核细胞递送白藜芦醇。
DOI：
10.2217/nnm-2018-0019
复制
发表时间：
2018-06-01
期刊：
影响因子：
5.5
通讯作者：
Guo, Xiuming
中科院分区：
文献类型：
--
作者：
Shen, Jieliang;Zhuo, Naiqiang;Guo, Xiuming
目的：目的制备一种新型超声介导的聚乳酸-羟基乙酸纳米泡（NBs）作为靶向给药手段，以提高椎间盘退变（IDD）的髓核细胞靶向治疗效果。材料与方法：采用复乳法制备白藜芦醇（RES）包埋纳米粒。使用碳二亚胺方法将活性NP细胞靶向生物标志物⑶ H 2抗体（AbCDH 2）进一步缀合至NB。然后，对RES/AbCDH 2纳米粒的物理性质、细胞靶向性、体外和体内抗肿瘤作用进行了研究。结果：RES/AbCDH 2纳米粒具有较高的RES负载效率，且超声可加速RES的释放。此外，RES/AbCDH 2 NB治疗在体外表现出优异的抗分解代谢能力;在IDD兔模型中，US介导的RES/AbCDH 2 NB注射有效地延缓了体内椎间盘的退行性过程。结论：RES/AbCDH 2纳米棒联合超声照射和药物递送可作为IDD的一种新的治疗选择。
Aim: To improve nucleus pulposus cell-targeted therapy for intervertebral disc degeneration (IDD) by fabricating a novel kind of ultrasound (US)-mediated poly(lactic-co-glycolic acid) nanobubbles (NBs) as a means of targeted drug delivery. Materials & methods: The resveratrol (RES)-embedded NBs were synthesized using a double-emulsion method. The active NP cell-targeting biomarker CDH2 antibody (AbCDH2) was further conjugated to the NBs using a carbodiimide method. Then, this RES/AbCDH2 NBs were examined by physical properties, specifc cell-targeting ability, anticatabolism effect in vitro and in vivo. Results: RES/AbCDH2 NBs exhibited high RES-loading efficiency, and US triggered accelerated RES release. Furthermore, RES/AbCDH2 NB treatment exhibited excellent anticatabolic ability in vitro; and in an IDD rabbit model, US-mediated RES/AbCDH2 NB injection effectively retarded the degenerative process of the intervertebral disc in vivo. Conclusion: The combination of US irradiation and drug delivery through RES/AbCDH2 NBs can be considered as a novel treatment option for IDD.