Membrane phospholipid asymmetry as a factor in erythrocyte-endothelial cell interactions.
Membrane phospholipid asymmetry as a factor in erythrocyte-endothelial cell interactions.
复制标题
膜磷脂不对称是红细胞-内皮细胞相互作用的一个因素。
DOI：
10.1002/jcp.1041230210
复制
发表时间：
1985
影响因子：
5.6
通讯作者：
Williamson,P
中科院分区：
文献类型：
--
作者：
Schlegel,RA;Prendergast,TW;Williamson,P
使用红细胞血影，将人红细胞粘附到血管内皮细胞的趋势评估为红细胞膜磷脂跨双层分布的函数，其中通过改变红细胞血影制备条件来操纵跨双层脂质排列。通过两种不同的测定，具有对称脂质双层的血影强烈粘附到培养的内皮细胞的单层上，而具有正常不对称膜的血影（如正常红细胞）则不然。这些结果提供了直接证据，表明磷脂不对称性的变化可以改变红细胞粘附内皮细胞的倾向，因此暗示跨双层磷脂排列可能影响红细胞在循环系统中的行为，并可能有助于微血管闭塞的形成。
The tendency of human erythrocytes to adhere to vascular endothelial cells was assessed as a function of the transbilayer distribution of the phospholipids of the erythrocyte membrane, using erythrocyte ghosts in which transbilayer lipid arrangement was manipulated by varying the conditions under which the ghosts were prepared. By two different assays, ghosts with symmetric lipid bilayers adhered strongly to monolayers of cultured endothelial cells, whereas ghosts with normal asymmetric membranes, like normal erythrocytes, did not. These results provide direct evidence that changes in phospholipid asymmetry can alter the tendency of erythrocytes to adhere to endothelial cells, and therefore imply that transbilayer phospholipid arrangement may influence the behavior of erythrocytes in the circulatory system and may contribute to the formation of microvascular occlusions.