Radiation-induced changes in cellularity and proliferation in human oral mucosa
Radiation-induced changes in cellularity and proliferation in human oral mucosa
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DOI：
10.1016/s0360-3016(01)02721-3
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发表时间：
2002-03-15
影响因子：
7
通讯作者：
Denham, JW
中科院分区：
文献类型：
--
作者：
Dörr, W;Hamilton, CS;Denham, JW
目的：量化头颈部肿瘤常规放疗期间口腔黏膜细胞密度和增殖率，并与口腔黏膜炎的临床评分进行比较。方法与材料：1996 ~ 1999年共纳入22例患者。在放疗前或放疗期间（5 x 2 Gy/周）进行粘膜活检。切片在切除后立即用氚化胸腺嘧啶体外孵育以标记dna合成细胞。结果：上皮细胞密度呈双相辐射反应。在第1周期间，细胞数量急剧下降至照射前的50%左右（1000个细胞/毫米上皮），随后在治疗结束时，细胞数量逐渐下降至约400个。初始阶段以抑制增殖为基础，第1周结束时标记细胞为5-10个/mm，而标记细胞为60个/mm。在控制。随后，以20个标记细胞/mm的速度部分恢复增殖。在放射治疗肿瘤组/欧洲癌症研究和治疗组织的0级（类似850个细胞/分钟）和2级（325个/毫米）或3级（370个/毫米）之间的细胞数量有显著差异。1级、2级和3级反应间无显著差异。结论：常规分割放疗在第1周诱导细胞生成快速抑制，导致细胞数量迅速减少。随后，增殖的部分恢复显著降低了细胞损失率。这些过程明显先于临床反应。当观察到最大的临床反应时，再生，定义为细胞的恢复，已经在进行中。在常规分馏中，临床反应分级与细胞密度测量的对应程度较差。(C) 2002爱思唯尔科学有限公司
Purpose: To quantify the oral mucosal cell density and proliferation rate during conventional radiotherapy of head-and-neck tumors and to compare these parameters with clinical scoring of oral mucositis.Methods and Materials: Between 1996 and 1999, 22 patients were included in this study. Mucosal biopsies were taken before or during the radiotherapy course (5 x 2 Gy/wk). Biopsies were incubated in vitro with tritiated thymidine immediately after excision to label DNA-synthesizing cells.Results: Epithelial cell density followed a biphasic radiation response. A steep decrease to about 50% of the preirradiation value (1000 cells/mm epithelium) during Week 1 was followed by a more gradual loss to about 400 cells at the end of treatment. The initial phase was based on the depression of proliferation, with 5-10 labeled cells/mm at the end of Week 1 vs. 60 labeled cells/mm. in controls. Subsequently, proliferation was partially restituted at 20 labeled cells/mm. A significant difference in cell numbers was seen between Radiation Therapy Oncology Group/European Organization for Research and Treatment of Cancer Grade 0 (similar to850 cell/min) and Grade 2 (325/mm) or Grade 3 (370/mm). No significant differences were observed between reaction grades 1, 2, and 3.Conclusion: Conventionally fractionated radiotherapy induces a rapid suppression in cell production in Week 1, which results in a prompt reduction in cell numbers. Subsequently, a partial restoration of proliferation significantly reduces the rate of cell loss. These processes clearly precede the clinical response. Regeneration, defined as restoration of cellularity, is already under way when the maximal clinical response is observed. Clinical reaction grading corresponds poorly to cellular density measures during conventional fractionation. (C) 2002 Elsevier Science Inc.