Studies on the microsomal electron-transport system of anaerobically grown yeast. I. Intracellular localization and characterization.
Studies on the microsomal electron-transport system of anaerobically grown yeast. I. Intracellular localization and characterization.
复制标题
厌氧生长酵母微粒体电子传输系统的研究。
DOI：
10.1093/oxfordjournals.jbchem.a130503
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发表时间：
1974
影响因子：
2.7
通讯作者：
R. Sato
中科院分区：
文献类型：
--
作者：
Y. Yoshida;H. Kumaoka;R. Sato
本文对厌氧培养的酵母细胞的细胞色素进行了研究，在10，000 × g和105，000 × g之间沉淀的部分中回收了细胞色素b_5和P-450，经化学分析和电子显微镜检查鉴定为酵母微粒体。这些来自微粒体的细胞色素被NADH或NADPH还原，还原的细胞色素容易被空气氧化。从这些结果可以得出结论，厌氧培养的酵母细胞微粒体含有与哺乳动物肝细胞微粒体相同的电子传递成分，即，细胞色素b 5、P-450、NADH-细胞色素b 5还原酶[EC 1.6. 2.2]和NADPH-细胞色素c还原酶[EC 1.6.2.4]。这些成分可能包含类似于肝微粒体的电子传递系统。
The cytochromes of anaerobically grown yeast cells were studied; cytochromeb5and P-450 were recovered in the fraction which was precipitated between 10,000 xgand 105,000Xgand were identified as yeast microsomes by chemical analysis and electron microscopic examination. These cytochromes from the microsomes were reduced by either NADH or NADPH and the reduced cytochromes were readily oxidized by air. Moreover, evidence for the presence of NADH-cytochromeb5reductase and NADPH-cytochromecreductase was obtained.From these results, it was concluded that microsomes of anaerobically grown yeast cells contain the same electron-transferring components as the microsomes of mammalian hepatocytes,i.e.,cytochromeb5, P-450, NADH-cytochromeb5reductase [EC 1.6. 2.2] and NADPH-cytochrome c reductase [EC 1.6.2.4]. These components may comprise an electron-transport system resembling that of hepatic microsomes.