Inter-Population Variability of Endosymbiont Densities in the Asian Citrus Psyllid (Diaphorina citri Kuwayama).
Inter-Population Variability of Endosymbiont Densities in the Asian Citrus Psyllid (Diaphorina citri Kuwayama).
复制标题
亚洲柑橘花园（diaphorina citri kuwayama）中内共生密度的人口间变异性。
DOI：
10.1007/s00248-016-0733-9
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发表时间：
2016-05
影响因子：
3.6
通讯作者：
Pelz-Stelinski KS
中科院分区：
文献类型：
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作者：
Chu CC;Gill TA;Hoffmann M;Pelz-Stelinski KS
亚洲柑橘木虱（Diaphorina citri Kuwayama）是一种能够传播北美柑橘绿化病原菌亚洲解放候选菌（Candidatus Liberibacter asiaticus, CLas）的害虫。柑橘还含有沃尔巴克氏菌（Wolbachia）、候选者Carsonella ruddii和候选者Profftella armatura三种内共生菌，它们可能会影响柑橘的生理和适应性。尽管对这些细菌进行了基因组研究，但关于它们在柑橘中的生态学和种群间变异仍不清楚。本研究利用定量PCR技术检测了不同种群成虫柑橘中各内共生菌的密度。在田间条件下，3种内共生菌的密度呈显著正相关，且与柑桔的性别和生境有关。此外，CLas的感染密度在不同人群中也存在差异。虽然一项汇集了不同种群的柑橘柑橘的分析表明，与未感染的个体相比，感染clas的个体往往具有较低的内共生生物密度，但当将种群作为一个因素纳入分析时，差异并不显著，这表明其他种群特异性因素可能对内共生生物密度有更强的影响。为了确定密度差异是否存在遗传基础，我们比较了在标准化实验室条件下饲养的两个柑桔种群中年龄为class阴性的雌性的内共生体密度。结果表明，沃尔巴克氏体感染密度的种群间变异与内共生菌或宿主的基因型有关。这项工作的发现有助于了解柑橘D. citri-bacterial associations，这可能有助于开发柑橘绿化管理方法，如预防CLas的传播。本文的在线版本（doi:10.1007/s00248-016-0733-9）包含补充材料，仅供授权用户使用。
The Asian citrus psyllid (Diaphorina citri Kuwayama) is an insect pest capable of transmitting Candidatus Liberibacter asiaticus (CLas), the causal agent of citrus greening in North America. D. citri also harbors three endosymbionts, Wolbachia, Candidatus Carsonella ruddii, and Candidatus Profftella armatura, which may influence D. citri physiology and fitness. Although genomic researches on these bacteria have been conducted, much remains unclear regarding their ecology and inter-population variability in D. citri. The present work examined the densities of each endosymbiont in adult D. citri sampled from different populations using quantitative PCR. Under field conditions, the densities of all three endosymbionts positively correlated with each other, and they are associated with D. citri gender and locality. In addition, the infection density of CLas also varied across populations. Although an analysis pooling D. citri from different populations showed that CLas-infected individuals tended to have lower endosymbiont densities compared to uninfected individuals, the difference was not significant when the population was included as a factor in the analysis, suggesting that other population-specific factors may have stronger effects on endosymbiont densities. To determine whether there is a genetic basis to the density differences, endosymbiont densities between aged CLas-negative females of two D. citri populations reared under standardized laboratory conditions were compared. Results suggested that inter-population variability in Wolbachia infection density is associated with the genotypes of the endosymbiont or the host. Findings from this work could facilitate understanding of D. citri-bacterial associations that may benefit the development of approaches for managing citrus greening, such as prevention of CLas transmission. The online version of this article (doi:10.1007/s00248-016-0733-9) contains supplementary material, which is available to authorized users.