Supramolecular methods: the 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) transport assay
Supramolecular methods: the 8-hydroxypyrene-1,3,6-trisulfonic acid (HPTS) transport assay
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DOI：
10.1080/10610278.2021.1999956
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发表时间：
2021-11-19
影响因子：
3.3
通讯作者：
Gale, Philip A.
中科院分区：
文献类型：
--
作者：
Gilchrist, Alexander M.;Wang, Patrick;Gale, Philip A.
利用8-羟基-1，3，6-三磺酸监测囊泡内部pH的脂膜转运法(HPTS法)是分析阴离子载体促进的磷脂膜转运活性的一种广泛使用的技术。本文描述了进行这种转运分析的分步技术，详细说明了使用这种方法来确定阴离子载体的活性和转运机制的优点和缺点。
The liposomal membrane transport assay, which uses 8-hydroxypyrene-1,3,6-trisulfonic acid to monitor the internal pH of the vesicles (the HPTS assay), is a widely used technique for analysing the activity of anionophore-facilitated transport across a phospholipid membrane. This paper describes the stepwise technique to conduct this transport assay, detailing both the perks and pitfalls of using this method to determine the activity of an anionophore and the transport mechanism.