Collagen I fiber density increases in lymph node positive breast cancers: pilot study
Collagen I fiber density increases in lymph node positive breast cancers: pilot study
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DOI：
10.1117/1.jbo.17.11.116017
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发表时间：
2012-11-01
影响因子：
3.5
通讯作者：
Glunde, Kristine
中科院分区：
文献类型：
--
作者：
Kakkad, Samata M.;Solaiyappan, Meiyappan;Glunde, Kristine
胶原I （Col1）纤维是人类乳腺癌细胞外基质的主要结构成分。在一项初步的试点研究中，我们探讨了原发性人类乳腺癌中Col1纤维密度与淋巴结转移发生之间的联系。用二次谐波显微镜（SHG）在有淋巴结转移（LN+）和没有淋巴结转移（LN-）的原发性乳腺癌患者中检测到Col1纤维。使用我们内部SHG图像分析软件量化的Col1纤维密度在LN+（纤维体积= 29.22% +/- 4.72%，纤维间距离= 2.25 +/- 0.45 μ m）的原发性人乳腺癌患者中显著高于LN-（纤维体积= 20.33% +/- 5.56%，纤维间距离= 2.88 +/- 1.07 μ m）患者。通过对Col1纤维的共现矩阵和傅里叶变换进行织构分析，证明LN+与LN-的共现矩阵、能量、纵横比和偏心率参数存在显著差异。我们还证明了组织固定和石蜡包埋对SHG Col1纤维检测和定量的影响可以忽略不计。原发性乳腺肿瘤中高Col1纤维密度与乳腺癌转移有关，可能作为转移的影像学生物标志物。(c) 2012年中国光学仪器工程师学会（SPIE）；(jbo.17.11.116017 DOI: 10.1117/1.)
Collagen I (Col1) fibers are a major structural component in the extracellular matrix of human breast cancers. In a preliminary pilot study, we explored the link between Col1 fiber density in primary human breast cancers and the occurrence of lymph node metastasis. Col1 fibers were detected by second harmonic generation (SHG) microscopy in primary human breast cancers from patients presenting with lymph node metastasis (LN+) versus those without lymph node metastasis (LN-). Col1 fiber density, which was quantified using our in-house SHG image analysis software, was significantly higher in the primary human breast cancers of LN+ (fiber volume = 29.22% +/- 4.72%, inter-fiber distance = 2.25 +/- 0.45 mu m) versus LN- (fiber volume = 20.33% +/- 5.56%, inter-fiber distance = 2.88 +/- 1.07 mu m) patients. Texture analysis by evaluating the co-occurrence matrix and the Fourier transform of the Col1 fibers proved to be significantly different for the parameters of co-relation and energy, as well as aspect ratio and eccentricity, for LN+ versus LN- cases. We also demonstrated that tissue fixation and paraffin embedding had negligible effect on SHG Col1 fiber detection and quantification. High Col1 fiber density in primary breast tumors is associated with breast cancer metastasis and may serve as an imaging biomarker of metastasis. (c) 2012 Society of Photo-Optical Instrumentation Engineers (SPIE). [DOI: 10.1117/1.JBO.17.11.116017]