Characterization of a full-length infectious clone of bovine foamy virus 3026
Characterization of a full-length infectious clone of bovine foamy virus 3026
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DOI：
10.1007/s12250-014-3382-5
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发表时间：
2014-04-01
影响因子：
5.5
通讯作者：
Qiao, Wentao
中科院分区：
文献类型：
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作者：
Bing, Tiejun;Yu, Hong;Qiao, Wentao
大多数泡沫病毒（FV）的生物学特性知之甚少，包括牛泡沫病毒（BFV）。BFV 3026株（BFV 3026）是从中国张家口的一头感染母牛的外周血单个核细胞中分离的。从BFV 3026感染的细胞中获得了BFV 3026的全长基因组克隆，其与美国分离的另一株BFV具有99%以上的氨基酸（AA）同源性。在转染到胎犬胸腺细胞中后，全长BFV3026克隆产生病毒结构和辅助蛋白、典型的细胞病变效应和病毒颗粒。这些结果表明，全长BFV 3026克隆是完全感染性的，可以用于进一步的BFV 3026研究。
The biological features of most foamy viruses (FVs) are poorly understood, including bovine foamy virus (BFV). BFV strain 3026 (BFV3026) was isolated from the peripheral blood mononuclear cells of an infected cow in Zhangjiakou, China. A full-length genomic clone of BFV3026 was obtained from BFV3026-infected cells, and it exhibited more than 99% amino acid (AA) homology to another BFV strain isolated in the USA. Upon transfection into fetal canine thymus cells, the full-length BFV3026 clone produced viral structural and auxiliary proteins, typical cytopathic effects, and virus particles. These results demonstrate that the full-length BFV3026 clone is fully infectious and can be used in further BFV3026 research.