A novel β1,3-N-acetylglucosaminyltransferase (β3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer
A novel β1,3-N-acetylglucosaminyltransferase (β3Gn-T8), which synthesizes poly-N-acetyllactosamine, is dramatically upregulated in colon cancer
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DOI：
10.1016/j.febslet.2004.11.037
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发表时间：
2005-01-03
期刊：
影响因子：
3.5
通讯作者：
Narimatsu, H
中科院分区：
文献类型：
--
作者：
Ishida, H;Togayachi, A;Narimatsu, H
采用计算机模拟方法鉴定了具有β 3-糖基转移酶基序的UDP-N-乙酰葡糖胺：β半乳糖β 1，3-N-乙酰葡糖胺转移酶（β 3 Gn-T）家族的一个新成员。这种新的beta3 Gn-T是从人结肠癌细胞系中克隆的，并根据其在系统发育树中的位置和酶活性命名为beta3 Gn-T8。β 3Gn-T8在体外将GlcNAc转移至四触角N-聚糖的Galbeta 1 -4GlcNAc的非还原末端。在流式细胞术分析中，用β 3 Gn-T8 cDNA转染的HCT 15细胞显示对莱亚和PHA-L4的反应性增加。这些结果表明，β 3 Gn-T8参与四触角（β 1，6-分支）N-聚糖上聚-N-乙酰乳糖胺链的生物合成。在大多数检查的结直肠癌组织中，β 3 Gn-T8转录物的水平显著高于正常组织。β 3 Gn-T8可能是参与结肠癌中β 1 -6支链N-聚糖上的聚-N-乙酰乳糖胺合成的酶。(C)2004年欧洲生物化学学会联合会。Elsevier B. V.出版，保留所有权利。
A new member of the UDP-N-acetvlalucosamine: beta galactose beta1,3-N-acetyliglucosaminyltransferase (beta3Gn-T) family having the beta3-glycosyltransferase motifs was identified using an in silico method. This novel beta3Gn-T was cloned from a human colon cancer cell line and named beta3Gn-T8 based on its position in a phylogenetic tree and enzymatic activity. beta3Gn-T8 transfers GlcNAc to the non-reducing terminus of the Galbeta1-4GlcNAc of tetraantennary N-glycan in vitro. HCT15 cells transfected with beta3Gn-T8 cDNA showed an increase in reactivity to both LEA and PHA-L4 in a flow cytometric analysis. These results indicated that beta3Gn-T8 is involved in the biosynthesis of poly-N-acetyllactosamine chains on tetraantennary (beta1,6-branched) N-glycan. In most of the colorectal cancer tissues examined, the level of beta3Gn-T8 transcript was significantly higher than in normal tissue. beta3Gn-T8 could be an enzyme involved in the synthesis of poly-N-acetyflactosamine on beta1-6 branched N-glycans in colon cancer. (C) 2004 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.