ANTIBIOTIC-RESISTANCE MUTATION IN 16S AND 23S RIBOSOMAL-RNA GENES OF ESCHERICHIA-COLI
ANTIBIOTIC-RESISTANCE MUTATION IN 16S AND 23S RIBOSOMAL-RNA GENES OF ESCHERICHIA-COLI
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DOI：
10.1093/nar/12.11.4653
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发表时间：
1984-01-01
影响因子：
14.9
通讯作者：
MORGAN, EA
中科院分区：
文献类型：
--
作者：
SIGMUND, CD;ETTAYEBI, M;MORGAN, EA
使用重组DNA和经典遗传学方法将壮观霉素抗性突变定位于16 S RNA基因的121个碱基对区域，将大环内酯-林可酰胺-链阳菌素B型抗性突变定位于23 S RNA基因的32个碱基对区域。这些区域的DNA序列分析表明，大观霉素耐药的结果从C/G到T/A转换的16 S RNA基因的位置1192。对大环内酯类、林可酰胺类和链阳菌素B型抗生素的耐药是由于23 S RNA基因2058位A/T到T/A的颠换所致。16 S RNA中的改变是在一个序列中，该序列可以参与替代碱基配对，这些替代碱基配对被认为参与了易位过程。23 S RNA中的改变鉴定了对肽基转移重要的序列。
Recombinant DNA and classic genetic procedures were used to map a spectinomycin resistance mutation to a 121 base pair region of a 16S RNA gene and a macrolide-lincosamide-streptogramin type B resistance mutation to a 32 base pair region of a 23S RNA gene. DNA sequence analysis of these regions revealed that spectinomycin resistance results from a C/G to T/A transition at position 1192 of a 16S RNA gene. Resistance to macrolide, lincosamide and streptogramin type B antibiotics resnlts from an A/T to T/A transversion at position 2058 of a 23S RNA gene. The alteration in 16S RNA is in a sequence that can participate in alternate base pairing arrangenents that have been proposed to be involved in the translocation process. The alteration in 23S RNA identifies sequences important to peptidyl transfer.