Genome-wide analysis reveals increased levels of transcripts related with infectivity in peanut lectin non-agglutinated promastigotes of Leishmania infantum
Genome-wide analysis reveals increased levels of transcripts related with infectivity in peanut lectin non-agglutinated promastigotes of Leishmania infantum
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DOI：
10.1016/j.ygeno.2009.01.007
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发表时间：
2009-06-01
期刊：
影响因子：
4.4
通讯作者：
Larraga, Vicente
中科院分区：
文献类型：
--
作者：
Alcolea, Pedro J.;Alonso, Ana;Larraga, Vicente
代谢环前鞭毛体在白蛉媒介肠道内发育后在血餐期间传播。通过花生凝集素凝集和差速离心从原环前鞭毛体和后环前鞭毛体的无菌培养物中分离婴儿利什曼原虫是有争议的。本研究的目的是同时分离两个馏分从同一人口在静止期的纯培养，并比较其表达谱的全基因组鸟枪DNA微阵列。发现的317个基因具有有意义的阶段特异性调节值，表明通过PNA凝集对后环前鞭毛体进行负选择在L中是可行的。可以分离婴儿和两种级分。该亚群上调半胱氨酸肽酶A和参与脂磷酸聚糖、蛋白磷酸聚糖和糖蛋白生物合成的几个基因，所有这些都与感染性相关。事实上，我们已经通过U937人细胞系感染实验证实了PNA（-）前鞭毛体的感染率增加。这些数据支持后环前鞭毛体与感染性相关，并且缺乏与PNA的凝集是该亚群的表型标记。(C)2009 Elsevier Inc. All rights reserved.
Metacyclic promastigotes are transmitted during bloodmeals after development inside the gut of the sandfly vector. The isolation from axenic Cultures of procyclic and metacyclic promastigotes by peanut lectin agglutination followed by differential centrifugation is controversial in Leishmania infantum. The purpose of this study has been to isolate both fractions simultaneously from the same population in stationary phase of axenic culture and compare their expression profiles by whole-genome shotgun DNA microarrays. The 317 genes found with meaningful values of stage-specific regulation demonstrate that negative selection of metacyclic promastigotes by PNA agglutination is feasible in L. infantum and both fractions can be isolated. This subpopulation up-regulates a cysteine peptidase A and several genes involved in lipophosphoglycan, proteophosphoglycan and glycoprotein biosynthesis, all related with infectivity. In fact, we have confirmed the increased infection rate of PNA(-) promastigotes by U937 human cell line infection experiments. These data support that metacyclic promastigotes are related with infectivity and the lack of agglutination with PNA is a phenotypic marker for this subpopulation. (C) 2009 Elsevier Inc. All rights reserved.