Axon and Schwann cell partnership during nerve regrowth
Axon and Schwann cell partnership during nerve regrowth
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DOI：
10.1097/01.jnen.0000171650.94341.46
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发表时间：
2005-07-01
影响因子：
3.2
通讯作者：
Zochodne, DW
中科院分区：
文献类型：
--
作者：
Chen, YY;McDonald, D;Zochodne, DW
周围神经的再生涉及雪旺细胞（SC）与再生轴突合作的重要贡献。我们在桥接横断大鼠坐骨神经的再生室中研究了新轴突和雪旺细胞之间的这种协作，这些新轴突和雪旺细胞注定要改革周围神经卡车。有一个高度亲密的“舞蹈”轴突之间，随后生长和增殖的SC。没有SC的轴突只能短距离生长，几乎所有的轴突突起都有相关的SC突起。当再生室通过外部接入端口注入局部丝裂霉素（一种有丝分裂抑制剂）时，SC增殖、迁移和随后的轴突再生显著减少。将层粘连蛋白添加到丝裂霉素中并不能逆转这种再生滞后，并表明SC提供的不仅仅是层粘连蛋白的合成。层粘连蛋白单独输注补充内源性层粘连蛋白，并促进第一SC，然后轴突再生。“错误的方式”错误的轴突与错误的SC过程，并更多的桥梁暴露于丝裂霉素，但较少的层粘连蛋白补充桥梁。后来，到21天，再生桥的有髓鞘轴突再增殖，但那些在早期时间点单独暴露于丝裂霉素的轴突投资有实质性损害。改造周围神经卡车涉及轴突和SC之间非常密切和亲密的关系，必须增殖和迁移，层粘连蛋白促进。
Regeneration of peripheral nerve involves an essential contribution by Schwann cells (SCs) in collaboration with regrowing axons. We examined such collaboration between new axons and Schwann cells destined to reform peripheral nerve trucks in a regeneration chamber bridging transected rat sciatic nerves. There was a highly intimate "dance" between axons that followed outgrowing and proliferating SCs. Axons without SCs only grew short distances and almost all axon processes had associated SC processes. When regeneration chambers were infused through an external access port with local mitomycin, a mitosis inhibitor, SC proliferation, migration and subsequent axon regrowth were dramatically reduced. Adding laminin to mitomycin did not reverse this regenerative lag and indicated that SCs provide more than laminin synthesis alone. Laminin infused alone supplemented endogenous laminin and facilitated first SC then axon regrowth. "Wrong way" misdirected axons were associated with misdirected SC processes and were more numerous in bridges exposed to mitomycin, but were fewer in laminin supplemented bridges. Later, by 21 days, there was myelinated axon repopulation of regenerative bridges but those exposed to mitomycin alone at early time points had substantial impairments in axon investment. Reforming peripheral nerve trucks involves a very close and intimate relationship between axons and SCs that must proliferate and migrate, facilitated by laminin.